霍山石斛原球茎的诱导及培养方式对原球茎增殖的影响.pdfVIP

安徽农业大学学报，2008，35(2)：258—261 Journal of Anhui A cultural University 霍山石斛原球茎的诱导及培养方式对原球茎增殖的影响 金 青，马绍望，洪萨丽，蔡永萍，林 毅 (安徽农业大学生命科学学院，合肥230036) 摘 要：以霍山石斛试管苗带节的茎段为材料，研究不同激素组合对霍山石斛原球茎诱导的影响，并比较4种 不同培养方式对霍山石斛原球茎增殖的影响。结果表明，生长物质2，4一D是霍山石斛原球茎诱导的关键因子，霍 山石斛原球茎诱导的适宜培养基为Knudson+2，4一D 0．1 mg·L +KT 0．05 mg·L～；霍山石斛原球茎增殖培养阶 段，主要采用液体振荡培养方式，适时转接于固体培养基上交替培养，调节原球茎生长状态，采用这种改进的培养 方式生产的原球茎可用于生物反应器的大规模培养。 关键词：霍山石斛；原球茎；诱导；培养方式；增殖 中图分类号：Q949．718．43；Q943．1 文献标识码：A 文章编号：l672—352X(2008)02-0258-04 Induction of protocorm—like bodies from Dendrobium huoshanense and effects of different culture methods on protocorm multiplication JIN Qing，MA Shao-jun，HONG Sa—li，CAI Yong—ping，LIN Yi (School of Life Science，Anhui Agricultural University，Hefei 230036) Abstract：Effects of plant hormone combinations on induction of protocorm—Iike bodies from stem segments of Dendrobium huoshanense in vitro were studied and effects of four different culture methods on protocorm muhiplica— tion were compared．The results showed that 2．4一D was the key factor for the indution of protoeorm—like bodies from Dendrobium huoshanense．the optimum medium for the indution was Knudson+2．4一D 0．1 mg·L +KT 0．05 mg·L‘。．The main culture method was shaking—bottle liquid culture．sometimes solid culture method was used dur— ing protoeorm multiplication for regulating protoeorm growth．The protoeorm —like bodiescultured by this improved ． method would be used for large—scale culture in 1)ioreactor． Key words：Dendrobium huoshanense；protoeorm —like bodies；induction；culture methods；multiplication 石斛属 (Dendrobium)为兰科第二大属，多年 斛的开发利用和可持续发展带来不利影响，因而对 生草本植物，是重要的常用中药材，含有多种生物