surface plasmon resonance - 复旦大学物理教学实验中心fudan .ppt

Surface plasmon resonance [1] Sabban, Sari. Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high- affinity FcεRI receptor (PhD thesis), (2011) The University of Sheffield Figure2. The workings of the SPR chip [1] Figure1. The configuration of an SPR chip[1] History Woods explaining the concept of surface plasma excitation in gratings in 1902 [2] . Kretschmann et al. proposed the practical means of excitation of SPR at the metal–dielectric interface in the prism based configuration in 1968 [3]. The first major application work utilizing SPR was reported in 1983 by Liedberg et al[4]. [2] R.W. Wood, On a remarkable case of uneven distribution of light in a diffraction grating spectrum, Phil. Mag. 4 (1902) 396–402. [3] E. Kretschmann, H. Raether, Radiative decay of non-radiative surface plasmon excited by light, Z. Naturforsh 23A (1968) 2135–2136. [4] B. Liedberg, C. Nylander, I. Lundstr¨om, Surface plasmon resonance for gas detection and biosensing, Sens. Actuators B 4 (1983) 299–304. Kretschmann configuration Fig. 3. Three-layer Kretschmann configuration for SPR excitation [5] [5] Gaurav Gupta, Jun Kondoh .Tuning and sensitivity enhancement of surface plasmon resonance sensor. Sensors and Actuators B 122 (2007) 381–388. Here, k0 =2π/λ0 is the wave vector in free space, λ0 the freespace wavelength, npthe refractive index of the prism, ns the fractive index of the dielectric sample, and εm the complex dielectric constant of the metal [5]. SPR setup Fig. 4. Schematic of prism-based SPR setup working in angular interrogation mode. LD: laser diode(670nm); P: polarizer; PD: photodiode; ADC: analog-to-digital converter [5]. [5] Gaurav Gupta, Jun Kondoh .Tuning and sensitivity enhancement of surface plasmon resonance sensor Sensors and Actuators B 122 (2007) 381–388. Result Fig. 5. Experimental and theoretical SPR reflection spectra. Dots for experimental spectra and solid line for theoretical spectra. Fig.7 Log Log pl