响应面法优化胃蛋白酶酶解瘤背石磺肌肉蛋白工艺参数.pdf

安徽农业大学学报, 2015, 42(3): 391-397 Journal of Anhui Agricultural University [DOI] 10.13610/ki.1672-352x009 网络出版时间：2015-4-24 14:39:58 [URL] /kcms/detail/34.1162.S1439.009.html 响应面法优化胃蛋白酶酶解瘤背石磺肌肉蛋白工艺参数 * 刁 亚，沈和定 ，程知庆，姚理想，吴 欣，杜文俊 (上海海洋大学省部共建水产种质资源发掘与利用教育部重点实验室，上海海洋大学水产与生命学院，上海 201306) 摘 要：为了优化胃蛋白酶酶解瘤背石磺肌肉工艺参数，以温度、pH 、时间和液固比为试验因子，以水解度 为响应值，在单因素试验的基础上，根据 Box-Behnken 中心组合设计试验原理，采用 4 因素 3 水平的响应面试验 设计确定最佳工艺条件。结果表明，最佳酶解工艺参数为温度 35 ℃，pH 1.5 ，时间2 h ，液固比93 mL·g-1 。经过验 证，实际水解度为 11.38%，与理论值的RSD 为 0.26%。 关键词：瘤背石磺；胃蛋白酶；酶解；响应面 中图分类号：TS201.21 文献标识码：A 文章编号：1672352X (2015)03039107 Optimizing conditions for enzymolysis of Onchidium struma mantle protein using pepsin by response surface methodology DIAO Ya, SHEN Heding, CHENG Zhiqing, YAO Lixiang, WU Xin, DU Wenjun (Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306) Abstract: To optimize the enzymolysis conditions of Onchidium struma mantle protein using pepsin, the ef- fects of enzymolysis temperature, pH value, enzymolysis time and liquid-solid ratio on the hydrolysis rate were determined using the response surface methodology (RSM). The enzymolysis conditions were investigated based on a 4-variable, 3-level Box-Behnken central composite design on the basis of single factor. Results showed that the optimum technological conditions were: enzymolysis for 2 h at 35 ℃ and pH1.5 with the liquid-solid rate of -1 93 mL·g . The actual hydrolysis rate was 11.38%, which was 0.26% of the theoretical value of RSD. Key words: Onchidium struma; peptin; enzymolysis; response surface methodology [1] 研究表明动物蛋白具有抗氧化[5-8] [9] 瘤背石磺（Onchidium struma ） ，又名海