类植物螯合肽的原核表达与zn2＋螯合能力分析①.pdf

( ) 绗 鍗风 鏈 瑗 鍗 澶 瀛 瀛 鎶 鑷劧绉戝鐗 骞 鏈 锛擄紪 锛戯紥 銆€銆€銆€銆€銆€銆€銆€銆€銆€ 銆€銆€銆€銆€銆€銆€銆€銆€銆€銆€銆€锛掞紣锛戯紨 锛戯紥 ( ) Vol矶帮紦锛栥€€No矶帮紤锛 JournalofSouthwestUniversit NaturalScienceEdition Dec矶般€€锛掞紣锛戯紨 y : / DOI 锛戯紣矶帮紤锛擄紬锛戯紭 矶癱nki矶皒dzk矶帮紥锛愶紤锛旐栋锛戯紥矶帮紣锛愶紩 j 绫绘鐗╄灟鍚堣偨鐨勫師鏍歌〃杈句笌 锛掞紜铻悎鑳藉姏鍒嗘瀽鈶 Zn 锛 锛 锛 锛 鏉滃皬鍏 , 缈熷啗楣 , 宕 钀 , 榄忓濞 , 銆€ 銆€ 銆€ 銆€ 锛 锛 锛 娼 瀹 , 鏉滄湪鑻 , 寮犲叴鍥 銆€ 銆€ 銆€ , ; , 锛戫栋瑗垮崡澶у 椋熷搧绉戝瀛﹂櫌 閲嶅簡 锛旓紣锛愶紬锛戯紩 锛掜栋瑗垮崡澶у 鍥壓鍥灄瀛﹂櫌 閲嶅簡 锛旓紣锛愶紬锛戯紩 : , , 鎽樿 鍙傜収閰舵硶鍚堟垚鐨勬鐗╄灟鍚堣偨搴忓垪 璁捐鍜屼汉宸ュ悎鎴愪簡绫绘鐗╄灟鍚堣偨鍩哄洜PC锛戯紤L锛 鐨勭紪鐮佸簭鍒 閲嶇粍杩 ( ) , , 鍚庡湪澶ц偁鏉嗚弻涓〃杈 缁 璇卞 鑳藉涓伴噺琛ㄨ揪鍑 绔甫鏈 铻嶅悎鑲界殑绫绘鐗╄灟鍚堣偨 pET锛擄紥a锛 IPGT N Trx PC锛戯紤L锛 锛掞紜 , , , H 涓斾娇澶ц偁鏉嗚弻瀵筞n 鐨勮灟鍚堣兘鍔涙彁鍗囦簡锛掜栋锛擄紩鍊 涓庡鐓х殑宸紓杈惧埌鏋佹樉钁楁按骞 琛ㄦ槑PC锛戯紤L锛鍏锋湁寰堝己鐨 锛掞紜 铻悎鑳藉姏 Zn 锛 锛掞紜 : ; ; 鍏 閿 璇 绫绘鐗╄灟鍚堣偨鍩哄洜 鍘熸牳琛ㄨ揪 铻悎鑳藉姏 銆€ 銆€ PC锛戯紤L锛 Zn 涓浘鍒嗙被鍙耳 鏂囩尞鏍囧織鐮? 鏂囩珷缂栧彿: ( ) Q锛楋紭锛栥€€銆€銆€銆€ A銆€銆€銆€銆€ 锛戯紪锛楋紦 锛欙紭锛栵紭锛掞紣锛戯紨锛戯紥 锛愶紣锛掞紩 锛愶紩 ( , ) , ( 妞嶇墿铻悎鑲 htochelatin 绠€绉 P