谷子逆境应答相关的钙依赖蛋白激酶基因sicdpk1 的克隆 - 作物学报.pdf

作物学报 ACTA AGRONOMICA SINICA 2014, 40(9): 15311539 / ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2014.01531 谷子逆境应答相关的钙依赖蛋白激酶基因SiCDPK1 的克隆与表达 余琴鸯 尹 恒 安利佳 李文利 , 116023 : CDPK , CDPK , RT-PCR cDNA CDPK , SiCDPK1 (GenBank KC249975.1) CDPK , 28 CDPK , CDPK 4 , SiCDPK1 II , 1596 bp, 531 , 59.5 kD, pI 5.94, CDPK , SiCDPK1 PEGABA : ; CDPK; ; Cloning and Expression Analysis of a Calcium-Dependent Protein Kinase Gene SiCDPK1 in Setaria italica * YU Qin-Yang, YIN Heng, AN Li-Jia, and LI Wen-Li School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116023, China Abstract: CDPK (calcium-dependent protein kinase) is a kind of essential calcium sensors and calcium responders, which plays important roles in response to various abiotic stresses in plants. In this research, we cloned the abiotic stress response related CDPK gene with RT-PCR in Setaria italica, designated as SiCDPK1 (GenBank accession number KC249975.1). We predicted 28 CDPKs in the Setaria italica genome by using the known Arabidopsis CDPK sequences as query sequences. The phylogenetic analysis showed the SiCDPK gene family was divided into four subgroups, in which SiCDPK1 belongs to subgroup II. The ORF of SiCDPK1 contains 1596 bp, which encodes 531 amino acids. The predicted protein molecular weight is 59.5 kD and pI is 5.94. SiCDPK1 has conserved protein domains of CDPK. The regulatory element analysis of promoter in SiCDPK1 showed a lot of cis-acting elements associated with different abiotic stresses. The RT