烟草废料中多酚类物质的浸提条件研究 - 南京师范大学图书馆电子阅览室.pdf

第20 卷第2 期 高 校 化 学 工 程 学 报 No.2 Vol.20 2006 年 4 月 Journal of Chemical Engineering of Chinese Universities Apr . 2006 文章编号：1003-9015(2006)02-0315-04 烟草废料中多酚类物质的浸提条件研究 , , , 李雪梅 陈育如 骆跃军 许晓风 (南京师范大学 生命科学学院 微生物工程重点实验室, 江苏 南京 210097) 摘 要：对烟草废料中多酚类物质的提取进行了探讨，对浸提溶剂的种类、料液比、浸提次数及浸提温度等条件进行 了优化，同时研究了微波、超声波等不同处理方法对浸提效果的影响。实验结果表明，以丙酮和水(1:2)作为浸提剂效 果较好，在料液比 1:9，温度 40 ℃，48h 的条件下，浸出液中多酚类物质的浓度为4.13 mg⋅mL− 1 。而用超声波处理时， 浸提时间缩短至 5 min，浸提液中多酚的浓度为 4.31 mg⋅mL− 1 ；在用微波处理时所需时间更短(90s) ，浸提液中的多酚类 浓度为 4.99 mg⋅mL− 1 。利用微波或超声波辅助处理，可以大幅度缩短浸提时间，提高浸提效率。 关键词：烟草废料；多酚；浸提；微波；超声波 中图分类号：X795 ；TQ028.96 文献标识码：A Study on Leaching Conditions of Polyphenols from Tobacco Residues LI Xue-mei, CHEN Yu-ru, LUO Yue-jun, XU Xiao-feng (Key Laboratory of Microbial Engineering, College of Life Sciences, Nanjing Normal University, Nanjing 210097, China) Abstract: The leaching of polyphenols from raw materials, such as tobacco residues, was studied. The leaching conditions, such as kinds of solvent, ratio of solid to liquor, leaching time and temperature, were optimized, and the effects of microwave and ultrasonic wave treatment on leaching were discussed. The results indicate that the 4.13 mg⋅mL− 1 of polyphenols can be obtained by using acetone and water (1:2) as preferred solvent (ratio of solid to liquor is 1: 9, 40°C, 48h). The concentration of polyphenols in solvent can be 4.31 mg⋅mL− 1 when leaching is processed with ultrasonic treatment and the leaching time can be shortened to 5min. The concentration of polyphenols in solv