不同金属离子对竹黄液体发酵的影响.pdf

• 164 • 中草药 Chinese Traditional and Herbal Drugs 第 42 卷 第 1 期 2011 年 1 月 不同金属离子对竹黄液体发酵的影响 项小燕，郑爱芳，谢 翎 安庆师范学院生命科学学院，安徽 安庆 246011 摘 要：目的 研究不同质量分数的 Cu2+ 、Fe3+ 、Cr3+和 Ca2+对竹黄菌液体发酵产竹红菌素量和菌丝体生物量的影响。方法 向培养基中添加不同质量分数的金属离子，在27 ℃、130 r/min 下培养 96 h 。结果 Cr3+ 的质量分数为0.005%时竹红菌素及 菌丝体生物量最大；Fe3+ 的质量分数为0.003%时菌丝体生物量最高，0.005%时竹红菌素量最高；Ca2+ 的质量分数为0.03%时 最有利于菌丝体生长，0.05%时最能促进竹红菌素的分泌；Cu2+ 的质量分数为 0.03%时生物量及竹红菌素量均达到最大值。 结论 添加金属离子后，菌丝体干质量最高达 15.28 g/L，竹红菌素量最高达 8.12 mg/g。 关键词：竹黄；竹红菌素；生物量；菌丝体；金属离子 中图分类号：R282.7 文献标志码：A 文章编号：0253 - 2670(2011)01 - 0164 - 03 Effect of different metal ions for submerged culture of Shiraia bambusicola XIANG Xiao-yan, ZHENG Ai-fang, XIE Ling School of Life Science, Anqing Teachers College, Anqing 246011, China Abstract: Objective To study the effect of different levels of Cu2+, Fe3+, Cr3+, and Ca2+ on mycelia biomass and the hypocrellin yield by submerged culture of Shiraia bambusicola. Methods The fungis were cultured under 27 ℃ and 130 r/min for 96 h with each level of metal ions. Results The biomass and the hypocrellin yield were the highest with Cr3+ of 0.005%; Fe3+ of 0.003% and 0.005% contents were favorable to the biomass and the hypocrellin yield, respectively; Ca2+ content of 0.03% was suitable for the mycelia growth and the hypocrellin yield benefited from the concentration of 0.005% ; Cu2+ content of 0.03% was added, both the biomass and the hypocrellin yield reached the best. Conclusion The highest mycelia dry weight and the hypocrellin yield could reach 15.28 g/L and 8.12 mg/g, respectively in addition of metal ion. Key words: Shiraia bambusicola P. Henn.; hypocrellin; biomass; mycelia; metal ions 竹黄 Shiraia bambusicola P. Henn.又名竹花、天 李加友等[10]从该菌的发酵液中得到了抗菌活 竹花、淡竹花、竹茧、赤团子、竹赤团子、竹赤斑 性成分；石贵阳等[11]对该菌液体发酵，从中得到竹 菌、竹参、竹三七、血三七等，属于真菌界子囊菌 红菌素的优化生长条件，而对于金属离子对液体发 亚门核菌纲肉座菌目肉座菌科竹黄属真菌[1] 。研究 酵