2型糖尿病患者泪液分泌及泪膜功能的初步研究.pdf

维普资讯 眼科研究 2004年 10月第 22卷第 5期 ·533· · 临床研 究 · 2型糖尿病患者泪液分泌及泪膜功能的初步研究 余 玲 谢汉平 孟晓红 王仕军 余延基 A studyontearsecretionandtearfilm functionintype2diabeticpatients YuLing，XieHanping，MengXiaohong，WangShqun，YuYa ．DepartmentofOphthalmology， AffiliatedSouth-westHospital，ThirdMilitaryUniversity，Chongqing400038，China Abstract 0bjective Tostudytearsecretionandtearfilmfunctionintype2diabeticpatients． Methods Tearfilm functiontestsincludingtearbreakuptime(BUT)，SchirmerItest(Slt)andRosebengal(RB)stainingwereperformedon25 diabeticpatientswith non—proliferativediabeticretinopathy (NPDR)and25diabeticpatientswithproliferativediabetic retinopathy(PDR)，and25healthypersonsservedascontrols．Totaltearproteinsdetectionandtearsodium dodecylsulfate polyacrylamidegelelectrophoresis(SDS—PAGE)weredonein10patientswithNPDR，10patienswithPDRand10controls． Results Compared with controlgroup，thevaluesofBUT and Sltwere decreased，and thepositivestainingrate ofRB was increased，andtheconcentrationoflactoferrin，lysozymeandtearspecificprealbuminweredecreasedindiabeticgroups，especially inPDR group．Therewasnosignificantdifferenceintotaltearproteinsamongthethreegroups． Conclusion Anabnomr alityof tearfilm functionintype2diabeticpatientsisf0und．andsevererchangesoccurindiabeticpatientswithPDR．SDS—PAGG isa usefultoolforscreeninganddetectingthetearabnormalitiesindiabeticpatients． Keywords diabetesmellitus；tearsecretion；tearfilm ；function 摘要 目的 探讨 2型糖尿病患者泪液分泌及泪膜功能。 方法 非增生型糖尿病视网膜病变 (NPDR)患者25例 (50眼)；增生型糖尿病视网膜病变(PDR)患者25例 (50眼)；正常对照25例 (50眼)。观察指标包括泪膜破裂时间、盂 加拉玫瑰红染色及 SchirmerI试验。NPDR组 、PDR组及对照组各 l0例 (10眼)测定泪液总蛋白和泪液主要蛋白质含 量。 结果 糖尿病组分别与对照组比较 ，SchirmerI测定值降低，泪膜破裂时间缩短，盂加拉玫瑰红染色阳性率增加 ， PDR患者尤其显著。泪液总蛋白含量三组间两两比较