现代生物学实验习题（Modern biology experiment）.docVIP

现代生物学实验习题（Modern biology experiment） The document comes from the network, it is my own collection organized, if have omitted, the error, still asks everyone to correct! Modern biology experiment (1) each experiment shall complete an experiment report in the format of experimental report paper (2) answer the relevant thinking questions in each module Summary of the questions: Part 1: biochemistry experiment First module: sugar experiment technology 1. How to choose the experimental materials in the biochemistry experiment? What is the standard? 2. What are the broken methods of materials? 3. Basic copper reagent method to determine whether the original sugar is direct titration or indirect titration? What are the advantages of the two methods of titration? 4. Which factors in the determination of crude fiber are the main factors affecting the determination results? Why do you have to strictly control the experimental conditions in the measurement of coarse fibers What is the result of this method? Second module: enzymatic experiment technique 1. Why do the specific experiments of enzyme make reagent test? What might be the result of omitting this step? 2. What are the specific characteristics of the enzyme? 3. The function characteristics of reversible inhibitors and irreversible inhibitors are illustrated 4. How does the inhibition of the three reversible inhibitors have to do with the substrate concentration? 5. What is the molecular mechanism of the loss of enzyme activity due to high temperature? How to overcome this effect in the separation and purification of enzymes? 6. What other factors can lead to decreased enzyme activity? 7. What are the molecular mechanisms that lead to the loss of enzyme activity? 8. The activity loss caused by the change of pH value, can the activity recover after pH recovery? 9. Try to explain the physical and biological significance of meters constant Km 10. Why is it that the meter constant Km is a characteristic constant of the enz