蓝莓根癌病发生调查及病原鉴定 - 吉林农业大学学报.PDF

吉林农业大学学 2011，04 Journal of Jilin Agricultural University E-mail:jlndxb@ 蓝莓根癌病发生调查及病原鉴定* 1 1 1 1 2 傅俊范 ，彭 超 ，严雪瑞 ，周如军 ，代汉萍 1. 沈阳农业大学植物保护学院，沈阳 110866 ；2. 沈阳农业大学园艺学院，沈阳 110866 摘 要：对辽宁、吉林、山东及黑龙江省的部分蓝莓苗圃及生产园中蓝莓根癌病发生情况、症状特点及危害程度进行了调 查，结果表明蓝莓根癌病多见于苗圃及酸度不适的生产园中。同时在上述地区采集到 57 份蓝莓冠瘿瘤和 10 份土壤样品， 经过菌株分离、回接寄主后获得 19 株致病菌。通过菌落形态、菌体特征、生理生化及 16S rDNA 序列比对，确定蓝莓根癌 病是由根癌土壤杆菌（Agrobacterium tumefaciens ）侵染所致。 关键词：蓝莓；根癌病；病原菌鉴定；16S rDNA 序列分析；根癌土壤杆菌 中图分类号：S663.9；S436.639 文献标识码：A 文章编号：1000-5684(2011) DOI: 网络出版地址： Investigation and Identification of Blueberry Crown Gall 1 1 1 2 FU Jun-fan, PENG Chao , YAN Xue-rui , ZHOU Ru-jun , DAI Han-ping 1. College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China; 2. College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China Abstract: The crown gall disease occurrence was investigated in different blueberry nurseries and fields located in Liaoning, Jilin, Shandong and Heilongjiang provinces. Fifty seven plant samples and ten soil samples were collected at the above areas. Nineteen of them showed pathogenicity through inoculating of healthy blueberry. The pathogen was identified as Agrobacterium tumefaciens by phenotypic features of the cells and colonies, biochemical characteristics and sequence analysis of 16S rDNA. Key words: blueberry; crown gall disease; identification of pathogen; 16S rDNA sequence analysis; Agrobacterium tumefaciens 植物根癌病由土壤杆菌（Agrobacterium sp. ）中 宁丹东、辽宁大连、吉林靖宇、山东泰安蓝莓种植园， 的致病菌株引起，是一种世界性的细菌病害，在欧洲