间充质干细胞长期传代培养条件的研究进展 - 中国细胞生物学学报.pdf

网络出版时间：2014-12-01 18:36 网络出版地址：/kcms/doi/10.11844/cjcb.2014.12.0177.html 中国细胞生物学学报 Chinese Journal of Cell Biology 2014, 36(12): DOI: 10.11844/cj cb.2014.12.0177 间充质干细胞长期传代培养条件的研究进展 彭学武 彭 健* (华中农业大学动物科技学院, 武汉430070) 摘要 间充质干细胞(mesenchymal stem cells, MSCs)是存在于成体组织间质部分的多能前体 细胞, 在体外具有自我更新增殖, 向成脂、成骨、成软骨分化的潜能, 在组织工程和细胞治疗方面 具有广阔的应用前景。MSCs在体外长期培养获得足够数量的细胞是MSCs应用的一个重要因素。 然而, 目前还没有建立MSCs长期传代培养的最适培养体系。该文将分别从培养体系中的基础培养 基、血清和生长因子对于MSCs细胞长期传代培养的影响进行论述, 旨在为建立MSCs体外长期传 代生长的最适培养体系提供理论依据。 关键词 间充质干细胞; 长期传代; 培养体系; 基础培养基; 血清; 生长因子 Research Progress in Culture Condition of Mesenchymal Stem Cells during Long-term Subculture Peng Xuewu, Peng Jian* (College of Animal Science and Technology, Huazhong Agriculture University, Wuhan 430070, China) Abstract Mesenchymal stem cells (MSCs) are multipotent precursors derived from the stromal fraction of adult tissues. MSCs possess the capacity of self-renewal and multipotency to differentiate into adipocytes, osteoblasts and chondrocytes, which give rise to promising application in tissue engineering and cell therapy. The long-term subculture to harvest high numbers of MSCs is one of the most crucial factors for the application. However, optimal culture system hasn’t been established so far. This paper will review the effect of basal medium, serum and growth factor of culture system on MSCs during the long-term subculture, respectively. The aim is to provide theoretical basis to establish optimal culture system f