17 392-395 工艺技术.4.20091208非专一性酶酶解壳聚 糖的最佳工艺研究4140A.pdfVIP

现代食品科技 Modern Food Science and Technology 2010, Vol.26, No.4 非专一性酶酶解壳聚糖的最佳工艺研究 毛新，赵力超，刘晓娟，叶惠仪 （华南农业大学食品学院，广东广州510642 ） 摘要：以甲壳低聚糖（COS ）平均相对分子质量为指标，研究纤维素酶以及以纤维素酶为主体与木瓜蛋白酶及果胶酶复 配水解壳聚糖的最佳工艺条件。结果表明影响纤维酶水解壳聚糖因素的主次是：pH温度 时间，最佳工艺条件为：pH=3.8,温 度=60 ℃,时间=2.5 h ，可得到Mr=1467.3的甲壳低聚糖。为了适合工厂生产需要，在pH=4.4 ，温度=60 ℃，时间=2.5 h条件下纤维素酶与木瓜蛋白酶及果胶酶复配，研究结果表明m(木瓜蛋白酶):m(纤维酶)=1:1时，甲壳低聚糖平均相对分子质量 为1000.1，m(果胶酶):m(纤维素酶)=1/3:1时，甲壳低聚糖平均相对分子质量达1134.4。 关键词：壳聚糖；甲壳低聚糖；纤维素酶；木瓜蛋白酶；果胶酶 文章篇号：1673-9078(2010)4-392-395 Optimization of Chitosan Degradation byNon-Specific Enzyme MAO Xin, ZHAO Li-chao ,LIU Xiao-juan, YE Hui-yi （College of Food Science, South China Agriculture University, Guangzhou 510642, China） Abstract: Using th chitooligosaccharides (COS) as quality indicators, the chitosan degradation by fiber enzyme was studied. The result showed that the influence order of some key factors was: pH temperature time. And the optimum degradation conditions were as follows: temperature 60 ℃, pH 3.8, and reaction time 2.5h, under which COS was achieved with its e average molecular weight of 1467.3. For industrial production of COS, the degradation conditions were determined as pH4.4, temperature 60 ℃and time 2.5 h. Under these conditions, the average molecular weight of COS was 1000.1 with the optimum ratio of papain to cellulose of 1:1. When the ratio of papain to cellulose was determined as 1 / 3:1, COS was achieved with its average molecular weight of 1134.4. Key words: chitosan; chitooligosaccharides; fiber enzyme; papaya enzyme; pectinase 392 壳聚糖是甲壳素的脱乙酰化产物，具有紧 U/mg ；远天43果胶酶，郑州市金水远天酶制 密的晶体结构，极大地限制了壳聚糖的应用 剂厂，酶活≥300 。将壳聚糖降解成分子量较小的小分子物质 U/mg ；乙酸钠，乙酸，冰乙酸，盐酸，氢氧 后，不仅有利于人体吸收，水解成的甲壳低 化钠，酒石酸钾钠，苯酚, 聚糖还具有很高的免疫调节功能及抗肿