4 445-447 下转489 基础研究 3+10 大蒜多糖对人体肠道中拟杆菌生长的影响.pdfVIP

现代食品科技 Modern Food Science and Technology 2010, Vol.26, No.5 大蒜多糖对人体肠道中拟杆菌生长的影响 曾艳华，陈玛宁，张宁，吴希阳，黄雪松 （暨南大学食品科学与工程系，广东广州510632 ） 摘要：本文通过添加大蒜多糖对人的粪悬液进行体外厌氧培养，采用选择性培养基对培养液的拟杆菌计数。并从培养液 中分离出两株拟杆菌菌株，通过七叶苷水解、糖发酵等生理生化试验，初步鉴定其为拟杆菌属（Bacteroides spp. ）。实验表明，大蒜多糖培养基中拟杆菌数量有明显增加，约增殖1.6~3.4个数量级，表明拟杆菌能够利用大蒜多糖促进自 身增殖。 关键词：大蒜多糖；拟杆菌；鉴定；肠道菌群 文章篇号：1673-9078(2010)5-445-447 Effect of Garlic Polysaccharides on the Growth of Bacteroides from Human Intestine ZENG Yan-hua, CHEN Ma-ning, ZHANG Ning, WU Xi-yang, HUANG Xue-song (Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China) Abstract: The effect of garlic polysaccharides on the growth of human faecal bacteria was investigated via in vitro fermentation under anaerobic conditions. Two strains of Bacteroides were isolated from the garlic polysaccharides fermentation broth with BBE selective culture plates. The two strains were classified as Bacteroides spp. according to Esculin hydrolysis, sugar fermentation and other physiological and biochemical tests. Results suggested the growth of Bacteroides can be significantly stimulated about 1.6~3.4 orders of magnitude by the four garlic polysaccharides and inulin. Key words: Garlic polysaccharides; Bacteroides ; identification; Gut bacteria 445 [13] 动物体内约定殖着400余种不同的微生物 1.1.2 发酵培养基（g/L ） ，现已证实动物粪便干重的1/3左右为细菌[1,2] 蛋白胨2 ，酵母浸膏2 ，NaCl 。拟杆菌是肠道中的优势厌氧菌，约占成年 0.1，K HPO 0.04，KH PO 2 4 2 4 [3] 0.04，MgSO •7H O 0.01，CaCl •2H O 个体肠道菌群的1/4以上 。近年来，国内外