ddヒト间叶系干细胞の 培养期间中の変化について c-myc.pdfVIP

YAKUGAKU ZASSHI 128(12) 1851―1856 (2008) 2008 The Pharmaceutical Society of Japan ―Articles― ヒト間葉系幹細胞のin vitro 培養期間中の変化について ―c-myc をターゲットとした遺伝子発現解析と染色体異常解析― 澤田留美，松岡厚子，松田良枝，土屋利江 Change in Characteristics of Human Mesenchymal Stem Cells during the In Vitro Culture ―c-myc Gene Expression and Chromosome Aberrations at the c-myc locus― AWADA  ATSUOKA ATSUDA SUCHIYA Rumi S , Atsuko M , Yoshie M , and Toshie T   Division of Medical Devices, National Institute of Health Sciences, 1 18 1 Kamiyoga, Setagaya-ku, Tokyo 158 8501, Japan ( ) Received July 23, 2008; Accepted September 3, 2008; Published online September 5, 2008 We investigated mRNA expression of c-myc and chromosome aberrations at the c-myc locus in the same passage number of human mesenchymal stem cells ( ) hMSCs . To understand the sensitivity of mRNA expression and the induc- tion of chromosome aberrations, we ˆrst tested them in hMSC and cancer cell lines ( ) HeLa S3, HOS, and OUMS-27 . The c-myc mRNA expressions in HeLa S3 and OUMS-27 were signiˆcantly higher than those in hMSC, but then those in HOS were not. On the other hand, c-myc aberrant cells detected by ‰uorescence in situ hybridization in HeLa S3, HOS, and OUMS-27 were signiˆcantly higher tha