双向电泳-质谱研究帕金森病人脑脊液诊断标志物 - 第三军医大学学报.doc

帕金森病人脑脊液生物标志物的蛋白质组学分析 陈莹1彭国光2余刚2 涂文斌3龙汉春3 江思德4 万金城4 (重庆医科大学附属第一医院神经内科 重庆 400016) 【摘要】目的：探讨采用比较蛋白质组学方法寻找帕金森病病人脑脊液中差异蛋白质作为诊断标志物。方法：随机纳入病人52名，分为帕金森病组27人（轻度14人、中度8人、重度5人）；疾病对照组16人；正常对照组9人，采用预冷丙酮处理样本，应用PH4-7胶条，12%聚丙烯酰胺凝胶进行双向电泳分离各组脑脊液蛋白质，运用PDquest8.0分析蛋白质电泳图谱，MALD I-TOF-MS质谱鉴定差异蛋白。结果：本实验展示了帕金森各病程脑脊液蛋白质图谱，发现了789±32个蛋白质点，其中4种蛋白质在帕金森各亚组与实验对照组，正常对照组比较中差异显著。结论：此四种差异蛋白质点存在于整个病程的帕金森病人的脑脊液中，可在帕金森病各病程阶段区别于与其他对照人群，有望成为帕金森病的诊断标志物。 【关键词】：帕金森病 ;蛋白质组学 ;诊断;质谱分析 ; The protemics about biomarker in cerebrospinal fluid of Parkinson’s disease patients CHEN Ying1，PENG Guo-guang2YU Gang2, TU Wen-bin3，LONG Han-chun3，JIANG Si-de4，WAN Jin-cheng4， Department of neurology, the First Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing 400016, China 【Abstract】Objective To discover the discrepant proteins in cerebrospinal fluid(CSF) of Parkinson’s disease(PD) patients and consider them as diagnotisc marker by comparative protemics.Methods to collect a crowd of 52 people randomized in all, set up a group combined of 27 PD pateients,which were devided in mild(14 patients)，moderate(8 patients) and severe (5 patients)；build the test control group(16 patients) and blank control group(9 patients).To deposit the proteins by ice-acetone and separate the proteins from CSF by two-dimensional electrophoresis technique with immobilized pH gradient strips(ph4-7) and 12%polyacrylamide.Then analyze the electrophorograms by PDquest8.0 and identificate the different protein points by MALD I-TOF-MS.Results This experiment acquire different electrophorograms of each period of PD and discover 789±32 protrin points. It is indicated that four proteins in PD groups have significant differences compared to test control group and blank control group.Conclusion：these four different protein points exsits in all course of disease, can distinguish the PD patients from the control groups in each stage and is promising be seem as diagnostic marker of PD . 【Keywords】:Parkinson’s dise