橄榄多酚含量测定方法的比较 - 食品科学.pdf

204 2014, Vol.35, No.08 食品科学 ※分析检测 橄榄多酚含量测定方法的比较 谢 倩，王 威，陈清西* （福建农林大学园艺学院，福建 福州 350002 ） 摘  要：采用酒石酸铁法、福林酚试剂法和紫外分光光度法3 种方法测定橄榄多酚类物质含量。结果表明： 3 种方法检出限及定量限的顺序为：紫外分光光度法＜福林酚试剂法＜酒石酸铁法，且3 者存在显著性差异 P （ ＜0 . 0 5 ）。3 种方法回收率顺序为：酒石酸铁法＞福林酚试剂法＞紫外分光光度法，但差异不显著 P （ ＞0.05 ），这3 种方法都具有良好的重复性。利用福林酚试剂法与紫外分光光度法测得的多酚含量显著高于酒 P P 石酸铁法（ ＜0.01 ），其中福林酚试剂法测得的多酚含量略高于紫外分光光度法，但差异不显著（ ＞0.05 ）。同 时，各方法测得的多酚含量存在良好的线性关系。实验证明紫外分光光度法灵敏度高、重复性好，且操作简单、快 速、经济，是一种较理想的测定橄榄多酚的方法。 关键词：橄榄；多酚；酒石酸铁法；福林酚试剂法；紫外分光光度法 Comparative Study on Three Different Methods for the Determination of Total Phenolics in Chinese Olive * XIE Qian, WANG Wei, CHEN Qing-xi (College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China) Abstract: Three analytical methods (ferrous tartrate colorimetry, Folin-phenol reagent method and UV spectrophotometry) were compared for the determination of total phenolics in Chinese olive in this study. The results showed the three methods followed the decreasing order: UV spectrophotometry ＜ Folin-phenol reagent method ＜ ferrous tartrate colorimetry by detection limit and quantitation limit with a significant difference among them (P ＜ 0.05), and ferrous tartrate colorimetry ＞ Folin-phenol reagent method ＞ UV spectrophotometry by recovery with the difference being not significant. All these three methods had good repeatability. The polyphenol content determined by Folin-phenol reagent method and UV spectrophotometry was significantly higher than that determined by ferrous tar