响应面优化丙酮粉法提取金银花多酚氧化酶.pdf

生物技术通报 ·研究报告· BIOTECHNOLOGY BULLETIN ( ) 2015, 31 7 :201-206 响应面优化丙酮粉法提取金银花多酚氧化酶 屈政  罗磊  杨彬  康新艳  （河南科技大学食品与生物工程学院，洛阳 471023） 摘 要 ： 对丙酮粉法提取金银花多酚氧化酶的工艺进行研究，结果表明，缓冲液pH、料液比、提取时间对PPO 比活力影响显 著。缓冲液pH 与料液比交互作用极其显著，回归模型拟合性良好 （ 2=99.31%）；影响显著顺序为 ：静置时间 缓冲液pH 值 料 R 液比 ；最优化工艺参数为 ：缓冲液pH7.5，料液比为 1 ∶120，提取时间为 14.5 min。在此条件下，酶比活力为137.389 U/mg。 关键词 ： 金银花 ；多酚氧化酶；丙酮粉；提取 ；响应面 DOI ：10.13560/ki.biotech.bull.1985.2015.07.031 Optimizing the Method of Acetone Powder to Extract Polyphenoloxidase from Honeysuckle Using Response Surface Methodology Qu Zheng Luo Lei Yang Bin Kang Xinyan （College of Food and Bioengineering ，Henan University of Science and Technology ，Luoyang 471023 ） Abstract: In this study, the method of acetone powder was optimized to extract the polyphenoloxidase（PPO）from honeysuckle flowers （ ）by response surface methodology. The results showed that three operating parameters had great effects on the PPO specific Lonicera japonica activity, and their effects were ranked as follows ：extraction time buffer pH material-to-liquid ratio. The established regression model was highly fitting（R2 = 99. 31%）, and the interaction effect between buffer pH and material-to-liquid ratio was extremely significant. The optimal extraction condition was pH 7. 49, material-to-liquid ratio 1 ∶120 and extraction time 14. 5 min, under which the PPO specific activity was 137. 389 U/mg. Key words: Lonicera japonica ；polyphenoloxidase ；acetone powder ；extract ；response surface 传统中医理论认为金银花具有清热解毒、疏 残留量较高，酶液纯度偏低。丙酮粉法主要是通过 利咽喉、消暑除烦的功效。新鲜金银花含水量约为