响应面法优化热回流提取光甘草定工艺研究 - new century science .pdf

/index.php/gjicmwm GJICMWM，2013,Vol.1,No.1 Extraction of glabridin with heat reflux extraction by response surface analysis DING Li-ying,LI Hong-yan Tianjin JiaShiTang Technology Co, Tianjin 300457, China Received: Nov 2, 2013 Accepted: Nov 21, 2013 Published: Dec 15, 2013 DOI: 10.14725/gjicmwm.v1n1a58 URL: /10.14725/gjicmwm.v1n1a58 This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Objective: To study the extraction process of glabridin from Glycyrrhiza glabra. Methods: The solid-liquid ratio, extraction time and extraction temperature are the three main factors in the experiment, and the extract solvent is ethyl acetate. Establish the response surface quadratic regression equation of glabridin extraction rate and purity. Results:The results indicate that the optimum conditions for glabridin are as follows: solvent is ethyl acetate, extraction time is 90 min, the ratio of solvent to solid is 35 ∶1 (ml/g), and extraction temperature is 44.7 ℃, purity and the maximum yield of Extraction of glabridin are 6.96% and 0.241%, The validation test shows that the experimental values of the model are 0.235% and 6.05% . Conclusion: The model equation can predict the experimental results, it can be used as reference in industrial production. Key words Glycyrrhiza glabra l; glabridin; response surface method; heat reflux extraction 响应面法优化热回流提取光甘草定工艺研究 丁利营，李红艳 天津嘉氏堂科技有限公司，天津 300457 通讯作者：丁利营 Email: dingly2009@126.com 【摘要】目的研究光果甘草中光甘草定的提取工艺。 方法 以乙酸乙酯为提取溶剂，选择料液比、提取时间和提取温度 3 个因素进行中心组合实验，建立光甘草定提取率和纯度的响应面，建立光甘草定提取率和纯度的二次回归方程。 结果 光甘 草定提取的最佳工艺条件为：乙酸乙酯为提取溶剂，提取时间 90min，提取温度44.7 ℃，料液比 1:35。该条件下光甘草定提 取率预测值为 0.241%，纯度为 6.96%，验证值为 0.235%与 6.05% 。 结论 所得模型方程能较好地预测实验结果，工业生产 中可应用参考。 【关键词】