罗非鱼碱性磷酸酶的化学修饰 - 广东农业科学.pdf

82 广东农业科学 2013 年第 3 期 罗非鱼碱性磷酸酶的化学修饰 1 2 武忠亮 刘纪宏 (1.兴义民族师范学院化学生物系袁 贵州 兴义 562400 曰 2.黔西南州畜牧水产局袁 贵州 兴义 562400) 要 采用 PMSF尧PCMB尧 NBS尧TNBS 尧SUAN 尧DTT 尧IAc尧 BrAc 等化学修饰剂 一定的条件下选择性修饰罗非鱼A LP 袁研 究罗非鱼ALP 分子中氨基酸侧链基团与酶活性中心的关系遥 结果表明院PMSF尧NBS 等两种化学修饰剂能显著抑制酶的活力袁而 PCMB尧TNBS尧SUAN 尧DTT 尧IAc尧BrAc 等对酶的活力影响不大遥 说明对丝氨酸残基尧色氨酸残基的化学修饰后对酶的活力影响很大袁 而酶分子中的其他功能基团不 酶的活性中心袁修饰后对酶的活力影响不大遥 关键词 罗非鱼曰 碱性磷酸酶曰 化学修饰 中图分类号 S965.125曰S912 文献标识码 A 文章编号 1004-874X 2013 冤03-0082-03 Chemical modification of alkaline phosphatase from Oreochromis niloticus 1 2 W U Zhong-liang , LIU J i-hong (1.De partment of Chemical Biology, X ingyi Normal College for Nationalities, X ingyi 562400, China; 2.Liv estoc k Hus bandry and A q uatic Products Management Bureau of Qianxinan A utonomous Prefecture, X ingyi 562400, China) Abstract: The alkaline phosphatase from Oreochromis niloticus (Tilapia) were selectively modified by chemical reagents such as PMSF, PCMB, NBS, TNBS, SUA N, DTT, IAc and BrAc respectively. It could be found that the enzyme activity was significantly decreased after modification of PMSF, NBS, but oppositely the PCMB, TNBS, SUAN, DTT, IAc, BrAc didn 爷t obviously effect the alkaline phosphatase enzyme activity. Studied of the chemical modification demonstrated that the Tryptophan residues and Serine residues were essential functional groups inside of the alkaline phosphatase active site, the same time suggested t