锌对不同发育时期子代果蝇基因组dna甲基化的影响 - 生物多样性.pdf

生物多样性 2012, 20 (6): 710–715 Doi: 10.3724/SP.J.1003.2012.06090 Biodiversity Science http: // 锌对不同发育时期子代果蝇基因组 DNA 甲基化的影响 郭欣欣 毛 雪 张 敏* (陕西师范大学生命科学学院, 西安 710062) 摘要: 为了初步探讨微量元素锌对不同发育阶段子代果蝇基因组DNA 甲基化的影响及可能机制, 以黑腹果蝇 (Drosophila melanogaster)Canton-S 品系为实验材料, 应用改良的AFLP分子标记技术分析了果蝇基因组DNA 甲基 化多态性, 以及不同浓度(2.5%、5%、10%)锌处理亲代果蝇后, 不同发育时期的子代果蝇基因组DNA 甲基化的改 变。结果显示: 胚胎、三龄幼虫、蛹期、雌及雄蝇DNA 甲基化多态条带比率均存在显著性差异; 亲本果蝇经锌处 理后, 其5–8 h胚胎DNA 甲基化多态条带比率在3个实验组中均显著高于对照组; 锌浓度为2.5% 时, 子代果蝇DNA 甲基化所受影响最大, 在5–8 h胚胎、12–15 h胚胎、18–21 h胚胎及雌蝇中, DNA 甲基化多态条带比率均显著高于对 照组。结果表明胚胎期DNA 甲基化多样性最高, 同时适当浓度锌处理, 可提高子代果蝇基因组DNA 甲基化多 态性。 关键词: 果蝇, 锌, AFLP, DNA 甲基化 Effect of zinc on DNA methylation diversity at different developmental stages of filial generation in Drosophila Xinxin Guo, Xue Mao, Min Zhang* College of Life Sciences, Shaanxi Normal University, Xi’an 710062 Abstract: To examine the features of DNA methylation diversity, we used a zinc treated Canton-S strain of Drosophila melanogaster as a model organism in this experiment. Our results showed significant differences in DNA methylation polymorphisms among embryos, third instar larvae, pupae, and female and male indi- viduals. After zinc treatment (at a concentration of 2.5%, 5%, and 10%) to the parental generation, the per- centage of DNA methylation polymorphic bands of offspring (5–8 h embryo) in the experimental groups F -1, F -2, F -3 were significantly higher than the control group. The experimental group F -2 (zinc concen- 1 1 1