膜分离制取可溶性菜籽蛋白及其对dpph 自由基的清除作用.pdf

148 2009, Vol. 30, No. 18 食品科学 ※工艺技术 膜分离制取可溶性菜籽蛋白及其对DPPH 自由基的清除作用 严梅荣，王丹丹，胡 蓉，鞠兴荣 (南京财经大学食品科学与工程学院，江苏省粮油品质控制及深加工技术重点实验室，江苏南京 2 10003) 摘 要：采用碱提取酸沉淀方法和膜分离技术从菜籽粕制取沉淀蛋白和可溶性蛋白，研究蛋白产物中的硫甙和植酸 分布、膜分离操作对可溶性蛋白的硫甙和植酸含量的影响、以及可溶性蛋白的抗氧化活性。结果表明，沉淀蛋白 得率较低，需要回收可溶性蛋白。菜籽粕中的硫甙被碱液提取并主要集中于可溶性蛋白，膜分离可以明显降低可 溶性蛋白中的硫甙含量，经CF=4 及DV=3 的膜分离，得到的可溶性蛋白中的硫甙含量仅为0.11mg/g 。可溶性蛋白 还具有清除DHPP 自由基活性的作用。碱液不能有效提取菜籽粕中的植酸，大部分植酸残留在提取蛋白后的残渣 中，膜分离难以分离可溶性蛋白中的植酸。 关键词：菜籽；可溶性蛋白；膜分离；抗氧化性 Membrane Separation for Preparation of Soluble Rapeseed Protein and Its Ability to Scavenge DPPH Free Radical YAN Mei-rong ，WANG Dan-dan ，HU Rong ，JU Xin g-rong (Key Laboratory of Grain and Oils Quality Control and Deep-utilizing Technology of Jiangsu Province, College of Food Science and Engineering, Nanj ing University of Finance and Economics, Nanj ing 2 10003, China) Abstract ：Alkali-soluble proteins extracted from rapeseed meal were fractionated into soluble and in soluble proteins by acid precipitation. The soluble protein was concentrated by ultrafiltration and dialyzed to remove anti-nutrients (such as glucosinolate and phytic acid) and inorganic salts. The distribution of glucosinolate and phytic acid in various rapeseed protein products and the effects of membrane separation and dialysis conditions on contents of glucosinolate and phytic acid in the soluble protein were studied, and the antioxidant activity of the soluble protein was evaluated by DPPH radical scavenging assay. The yield of the precipitated protein was low. So it was necessary to recover the soluble protein. The glucosinolate in rapeseed meal was extracted together with alkali-soluble proteins and was mainly contained in the soluble protein fraction. The content of glucosinolate