厌氧真菌发酵液中木聚糖酶活力的初步研究 - 南京农业大学学报.pdf

南京农业大学学报 　2003 , 27 ( 1) : 120～123 　 Journal of Nanjing Agricultural University 厌氧真菌发酵液中木聚糖酶活力的初步研究 朱崇淼, 毛胜勇, 孙云章, 朱伟云 (南京农业大学动物科技学院 , 江苏 南京 210095) 摘要 : 研究了厌氧真菌木聚糖酶粗酶的提取方法及其性质。结果表明 , 在 20 ℃, 厌氧真菌木聚糖酶粗酶的硫酸铵最佳 盐析饱和度为 70 % 。45 ℃烘干得到木聚糖酶粗酶制剂 , 该制剂比活力为 7 562 U ·g - 1 。对其性质的初步研究表明 , 该酶 的最适温度为 50 ℃, 最适pH 值为 50～60 。此外 , 其酶活性在 37～50 ℃、pH40～110 范围内较稳定。 关键词 : 厌氧真菌 ; 木聚糖酶 ; 酶学性质 中图分类号: S8526 　　　文献标识码 : A 　　　文章编号 : 1000 2030 (2004) 01 0120 04 Preliminary studie s on xylana se activity in fermentation supernatant from an anaerobic fungus ZHU Chongmiao , MAO Shengyong , SUN Yunzhang , ZHU Weiyun (College of Animal Science and Technology , Nanjing Agricultural University , Nanjing 210095 , China) Abstract : Xylanase from anaerobic fungal cultures was extracted using (NH ) SO and the enzyme characteristics were analyzed. The re 4 2 4 sults showed that the optimal extracting concentration for saturated (NH ) SO was 70 % at 20 ℃. After extraction and drying at 45 ℃, 4 2 4 its specific activity was 7 562 U ·g - 1 . The optimal temperature for xylanase activity was 50 ℃and optimal pH was in the range of 50 60. Xylanase activity was stable both in the range of 37 50 ℃and pH 40 to pH 110. Key words : anaerobic fungi ; xylanase ; enzyme characteristics ( ) 木聚糖酶 xylanase , EC3218 能特异性地降解木聚糖 , 其产物主要为木寡糖、木糖及阿拉伯糖 ( ) 等 , 由