蔓性千斤拔组织培养中种子灭菌和芽增殖的研究.pdfVIP

安徽农业科学，JournalofAnhuiASh．Sci．2008，36(32J：13981—13983 责任编辑 罗芸 责任校对 李洪 蔓性千斤拔组织培养中种子灭菌和芽增殖的研究 刘芳，韦鹏霄，岑秀芬，黄浩2*，余丽莹 (1．广西大学农学院，广西南宁53ooo5；2．广西药用植物园，~／ 530023) 摘要 [目的]研究蔓性千斤拔组织培养中种子灭菌的方法，筛选适宜芽增殖的外植体及激素组合。[方法]用乙醇、次氯酸钠和升汞的 不同组合对种子进行灭菌，然后比较顶芽和带腋芽的茎段作为外植体进行继代增殖的效果，探讨不同激素组合对芽增殖的影响。[结 果]较好的种子灭茵方法为：75％乙醇30s+10％NaC1010min十0．1％HgC15min；带腋芽的茎段为较好的继代增殖外植体。6-BA对 芽增殖有显著促进作用；KT对芽的增殖效果不如6．BA；芽增殖的最佳激素组合为6-BA2．0ms／L+I从 0．1ms／L，增殖倍数为6．74。 [结论]获得了较好的种子灭菌方法，筛选出适合继代增殖培养的外植体和激素组合，为蔓性千斤拔组织培养的深入研究提供实验基础。 关键词 蔓性千斤拔；组织培养；种子灭茵；芽增殖 中图分类号 S567．239 文献标识码 A 文章编号 o517—66l1(20O8)32—13981—03 StudyonSeedSterilizationandBudProliferationin．rissueCultureofFlemlngiaphi~pincrisisMerr．etRolf LIUFangetal (CollegeofAgronomy，GuangxiUniversity，Nanning，Guangxi530005) Abstract 0『biectiveITheaimoftIIispaperwastostudythemethodofseedsterilizationandtoselecthtesuitableexplantandhormoneCOB． binationforbudpmliferationintissuecultureofFlemingphilippinensisMerr．etR0lf．1Mehtod]Differentcombinationsofethanol，NaC10 andHgC1，were usedofrseedsterilization．htenhtecomparisonbetweenapicalbudandstem withaxitlarybudsasexplantforsubculturewas conducted．Finally，theeffectsofdifferenthormonecombinationsonbudproliferationwerediscussed．『Result]Thebetterseedsterilization mehtodof philippinensiswas 75％ ethanol30s+10％ NaC1010min+0．1％ HgC1，5min．Th eoptimalexplantofrsubcuhureandmulti． plicationwasstemwithaxillarybuds．6．BAhadsignificantpositiveeffectonbudproliferationof philippinensiswhiletheeffectofKTonbud proliferationwas poorerthan6．BA．Theoptimumhormonecombinationofrbudproliferationof philippinensiswas6．BA2．0ms／L +IAA O．1ms／Landpreliferationmultipleofbudsachieved6．74．C『onclusion]Theseedsterilizationmethodof philippinensiswasestablishedand thesuitbaleexplantandhormonecombinationofrbudproliferationwere screened．whichofferedthefoundationoffurtherstudyontissuecul— tureof philippinensis． Keywords FelmingiaphilippinensisMerr．etRoff；Tissueculture；Seedsterilization；Budp