脾虚证多发性抽动症小鼠模型的建立及评价 - 环球中医药杂志.pdf

环球中医药2016年9月第9卷第9期摇 Global Traditional Chinese Medicine,September2016,Vol郾9,No郾9 1摇055 ·论著 · 脾虚证多发性抽动症小鼠模型的建立及 评价 陈自佳摇 于文静摇 王素梅 揖摘要铱摇 目的摇 建立脾虚证多发性抽动症小鼠动物模型,初步了解该脾虚模型的一般特征。 方 法摇 腹腔注射亚氨基二丙腈350 mg/ (kg·d)结合浓度为1g/ mL 的番泻叶颗粒剂溶液20 mL/ (kg·d) 灌胃,连续8天,建立脾虚证多发性抽动症小鼠模型。 通过自主活动计数、刻板运动评分、体质量、食 量、大便粒数、胸腺及脾脏指数、胃泌素、D鄄木糖等指标对脾虚证多发性抽动症小鼠进行评价。 结果 摇 模型组、脾虚组小鼠造模后在相同的时间内活动路程及平均速度较空白对照组明显增加 (P0.05),体质量增加缓慢。 脾虚组小鼠的食量降低,但差异无统计学意义(P0.05)。 胸腺指数、 脾脏指数及胃泌素、D鄄木糖浓度均无统计学差异(P0.05)。 结论摇 本模型抽动症状稳定,具有脾虚 证的某些症状,一定程度上具备了脾虚证多发性抽动症的表面效度。 揖关键词铱摇 脾虚证;摇 多发性抽动症;摇 模型评价 揖中图分类号铱摇 R285.5摇 揖文献标识码铱摇 A摇 doi:10郾3969/ j郾issn郾1674鄄1749郾2016郾09郾006 Establishment and preliminary evaluation of a mouse model of tourette蒺s syndrome(TS) with spleen deficiency syndrome摇 CHEN Zi鄄jia, YU Wen鄄jing, WANG Su鄄mei. Paediatric Department, Dongfang Hospital of Beijing University of Chinese Medicine,Beijing 100078,China Corresponding author:WANGSu鄄mei,E鄄mail:wangsumei120@163.com 揖Abstract铱摇 Objective摇 To establish an animal model of TS in mouse with spleen deficiency syndrome,and to explore the general characteristics of the model of spleen deficiency. Methods摇 Mice were intraperitoneal injected iminodipropionitrile 350 mg/ (kg ·d) and intragastric administration Senna granules solution 20 mL/ (kg ·d) for 8 days. The independent activity counts, stereotyped movement score, body weight, food intake, feces granu, thymus and spleen index, gastrin, D鄄xylose and other indexes were used to evaluate TS mouse of spleen deficiency. Results摇 After modeling, the activity distance and average speed of model group and spleen deficiency group was significantly increased,and body weight increase slowly. Food intake of spleen deficiency group was decreased(P0.05). Thymus index,spleen index,gastrin and D鄄xylose concentration had no significant difference. Conclusion摇 The TS model has stable symptoms and s