负载王不留行黄酮苷纳米纤维作为创伤敷料的研究 - 生物医学工程学杂志.pdfVIP

• 394 • Journal of Biomedical Engineering, Jun. 2017, Vol. 34, No.3   ·论 著· 负载王不留行黄酮苷纳米纤维作为创伤敷料 的研究 1 1 1 2 2 邱玉宇 ，蔡维维，邱丽颖，王清清，魏取福 1. 江南大学 无锡医学院（江苏 无锡   214122 ） 2. 江南大学 纺织服装学院 生态纺织教育部重点实验室（江苏 无锡   214122 ） 【摘要】   本文采用静电纺丝制备了负载王不留行黄酮苷的聚乙烯醇苯乙烯吡啶盐（PVA-SbQ ）/玉米醇溶蛋 白（Zein ）纳米纤维，对纳米纤维的表面形貌和结构进行了观察，并对其进行了生物相容性评价。将负载王不留行 黄酮苷的 PVA-SbQ/Zein 纳米纤维作为实验组，以凡士林纱布为对照组，建立小鼠皮肤损伤模型，对两种敷料在 小鼠皮肤上的修复作用进行组织切片观察。实验结果表明，成功制备了负载王不留行黄酮苷的纳米纤维并呈现 良好的形貌；负载黄酮苷的 PVA-SbQ/Zein 纳米纤维对 L929 细胞无毒害作用，且黄酮苷的存在可以促进细胞黏 附生长。基于 PVA-SbQ/Zein/黄酮苷纳米纤维膜的创伤愈合材料对小鼠伤口愈合的效果要优于凡士林纱布。 【关键词】  静电纺丝；聚乙烯醇苯乙烯吡啶盐；玉米醇溶蛋白；纳米纤维；创伤敷料 Preparation and study of vaccarin-loaded nanofibers used as wound healing material 1 1 1 2 2 QIU Yuyu , CAI Weiwei , QIU Liying , WANG Qingqing , WEI Qufu 1. Wuxi Medical School, Jiangnan University, Wuxi, Jiangsu 214122, P.R.China 2. Key Laboratory of Eco-Textile, Ministry of Education, Jiangnan University, Wuxi, Jiangsu 214122, P.R.China Corresponding author: WEI Qufu, Email: qfwei@jiangnan.edu.cn 【Abstract 】 A drug vaccarin loaded polymer poly (vinyl alcohol) (PVA)-stilbazole quaternized (SbQ)/Zein was prepared in this study, using co-electrospun method. Then the morphologies and structures of PVA-SbQ/Zein composite nanofibers were observed by scanning electron microscope (SEM) and Fourier transform infrared spectrum (FTIR), respectively. Finally, biocompatibility of PVA-SbQ/Zein nanofibers with drug and without drug was evaluated. Results showed that vaccarin-loaded PVA-SbQ/Zein nanofibers had smooth surface and showed non-toxic to L929 cells. Drug vaccarin could promote cells attachment on nanofibers. The wound healing performance was examined in vivo by rat skin models and histological observations, and PVA-SbQ/Zein/vaccarin nanofibers showe