紫菀根的结构与主要药用成分积累研究 - 热带亚热带植物学报.pdfVIP

热带亚热带植物学报 2017, 25(1): 98 ~ 104 Journal of Tropical and Subtropical Botany 紫菀根的结构与主要药用成分积累研究 1,2 1 1 1 1 1,3* 郭伟娜 , 王蓉 , 黄力 , 程磊 , 刘耀武 , 方成武 (1. 安徽省中医药科学院亳州中医药研究所，安徽 亳州 236800; 2. 亳州职业技术学院药学院，安徽 亳州 236800; 3. 安徽中医药大学, 安徽 合肥230031) 摘要：为揭示紫菀根的结构、主要药用成分积累部位和含量，用石蜡切片法研究不同发育阶段根的结构、组织化学法定位三 萜皂苷和黄酮类成分的积累部位、HPLC 法测定根中紫菀酮、槲皮素和山奈酚的含量。结果表明，紫菀根的初生结构包括表 皮、皮层和维管柱。次生结构包括外皮层、皮层和维管组织，其中分泌道位于皮层内侧，数量与韧皮部束一致，随着根的增 粗，中央分化出髓部。三萜皂苷成分在韧皮部和皮层内侧积累较多；黄酮类成分积累于皮层和髓部。紫菀根下部的紫菀酮含 量高于上部，槲皮素和山萘酚仅为上部的 1/3。因此，建议加工时保留下部细根，实现资源综合利用。 关键词：紫菀；根；紫菀酮；槲皮素；山萘酚 doi: 10.11926/jtsb.3621 Studies on Structure and Accumulation of Main Chemical Constituents of Aster tataricus L. f. Roots 1,2 1 1 1 1 1,3* GUO Wei-na , WANG Rong , HUANG Li , CHENG Lei , LIU Yao-wu , FANG Cheng-wu (1. Bozhou Institute of Chinese Medicine, Anhui Academy of Chinese Medicine , Bozhou 236800, Anhui, China; 2. School of Pharmacy, Bozhou Vocational Techical College, Bozhou 236800, Anhui, China; 3. Anhui University of Chinese Medicine, Hefei 230031, China) Abstract: In order to reveal structure, accumulation parts and contents of chemical constituents in Aster tataricus L. f. roots, the root structure at different developmental stages were observed by paraffin section, the accumulation parts of triterpenoid saponins and flavonoids were located by histochemistry, and the their contents were determined by HPLC. The results showed that the primary root of A . tataricus could be divided into epidermis, cortex and stele, and the secondary root included exodermis, cortex and vascular tissue. The secrete channels located inside the cortex, which number was consistent with the phloem bundles. The center of root differentiated