大豆疫霉菌、水杨酸和创伤诱导的拟南芥蛋白质谱的比较研究 - 生物通.docVIP

大豆疫霉菌、水杨酸和创伤诱导的拟南芥蛋白质谱的比较研究 孙果忠，朱振东，武小菲，崔友林，王晓鸣 （中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程，北京 100081） 摘要：【目的】系统获得抗性（system acquired resistance，SAR）是植物抵抗病原菌侵染的一种复杂机制，本研究旨在鉴定大豆疫霉菌（Phytophthora sojae）诱导的拟南芥系统获得抗性相关的蛋白质。【方法】利用双向电泳分离创伤、水杨酸（Salicylic acid，SA）和大豆疫霉菌诱导的拟南芥叶片的总蛋白，并对28个差异表达的蛋白点进行了MALDI-TOF质谱鉴定。【结果】这28个蛋白点的表达模式可分为3种类型：13个点的表达随着伤口、SA和 P.sojae的诱导而上调；11个点的表达随着伤口、SA和 P.sojae的诱导而下调；4个点在伤口和P.sojae处理中的表达行为与SA处理相反。这些蛋白质涉及了物质代谢酶、调节因子或分子伴侣、氧化还原酶、防御相关蛋白、蛋白激酶、叶绿体蛋白和功能未知蛋白等复杂的生物学功能。【结论】创伤、SA和P.sojae诱导的拟南芥系统获得抗性涉及的抗性组分有很大的重叠，在信号传导网络上也存在一定的交叉。蛋白质组分析为研究复杂的植物抗性机制提供了可行的技术手段。 关键词：拟南芥；大豆疫霉菌；水杨酸；系统获得抗性；蛋白质组学 Comparative Proteomics of Arabidopsis Induced by Salicylic Acid, Wound and Phytophthora sojae SUN Guo-zhong, ZHU Zhen-dong, WU Xiao-fei, CUI You-lin, WANG Xiao-ming (Institute of Crop Science, Chinese Academy of Agricultural Sciences/The National Key Facilities for Crop Genetic Resources and Improvement, Beijing 100081) Abstract: 【Objective】System acquired resistance (SAR) is a complex disease resistance mechanism in plants. The study was aimed at elucidating the protein profiles of the SAR of Arabidopsis induced by Phytophthora sojae. 【Method】Total proteins prepared from Arabidopsis leaves induced by wound, salicylic acid (SA) and P. sojae were isolated by 2D PAGE. A total of 28 spots differentially expressed between the CK and treatment were identified by MALDI-TOF mass spectrometric analysis. 【Result】Among them, 13 spots were up regulated and 11 spots were down regulated in the treated leaves; interestingly, the expression patterns of 4 spots in leaves induced by SA were opposite to that of wound and P. sojae. Functions of these proteins could be classified as metabolic enzymes, chaperone, oxidoreductase, defense-related proteins, protein kinase, chloroplast proteins and unknown function proteins. 【Conclusion】Studies of expression patterns of these proteins have provided new information on the overlap of defense element and cross-communicating signal transduction pathw