翡翠贻贝足蛋白作为生物黏合剂的提取及应用 - core.pdf

27 6 Vol. 27 No.6 2010 12 Journal of Biomedical Engineering December 2010 * 1 1 1 1, 2 蒋 臻 刘加鹏 金利华 张其清 1( 厦门大学 材料学院生物材料系;厦门大学 生物医学工程研究中心, 福建省生物医学工程重点实验室; 厦门市生物医学工程技术研究中心,厦门361005) 2 ( 中国医学科学院 中国协 医科大学 生物医学工程研究所, 天津市生物医学材料重点实验室, 天津 300192) : , ( Pvfp) , Pvfp, ,(QCM), TM QCM , Pvfp CellTak ; T M , Pvfp CellT ak PLL(polyLlysine); ela 293T , Pvfp ; , Pvfp ; , Pvfp, Pvfp , : ; ; ; Q78;Q819 A 2010 Extraction and Application of Perna virid is Foot rotein as Bioadhesive 1 1 1 1, 2 Jiang Zhen Liu Jiapeng Jin Lihua Zhang Qiqing 1 (Research Center of B iomedical Eng ineering, X iamen University , K ey L aboratory of Biomedical E ngineer ing of Fuj ian, echnology R esearch Center of B iomedical Engineering of X iamen, X iamen 361005, China) 2 (Institute of Biomedical Eng ineering, Chinese Academy of Medical Science, Peking Union Medical College, K ey Laboratory of Biomedical Mater ial of ianj in, ianj in 300192, China) Abstract: Mussel adhesive proteins have attracted increasing interests for their potential use as environmentally friendly bioadhesives in medicine and aqueous conditions. In this study, surface coating analysis, quartz crystal mi crobalance ( QCM) , cell and bone tissue adhesion and cytotoxicity assay were used to study the properti