基于itraq 技术的加拿大一枝黄花提取物作用下铜绿微囊藻细胞差异 .pdfVIP

中国环境科学 2015,35(6) ：1822~1830 China Environmental Science 基于 iTraq 技术的加拿大一枝黄花提取物作用下铜绿微囊 藻细胞差异表达蛋 黄莹莹 1, 白 羽 1,王 艳 1,2*,孔海南 1 (1.上海交通大学环境科学与工程学院,上海 200240 ；2.上海交通大学公 共卫生学院,上海 200025) 摘要：为研究加拿大一枝黄花提取物对藻类生长抑制作用的分子机理,应用 iTraq 技术结合 LC-MS-MS,分析了铜绿微囊藻细胞蛋白 质的差异表达 .共鉴定出 261 种差异蛋白(变化倍数≥ 1.5),其中表达上调的 220 种,表达下调的 41 种. GO 功能分类显示,加拿大一枝黄 花提取物通过影响细胞代谢过程、蛋白质催化和结合功能等方式抑制藻细胞生长.KEGG 通路分析表明,通路大类中富集程度位于前 3 位的均是代谢通路,包括能量代谢、碳水化合物代谢和氨基酸代谢,通路中富集程度最高的是糖酵解/糖异生通路 .本研究发现了加拿 大一枝黄花提取物对藻细胞生长抑制作用的分子靶点,为从分子层面阐明其抑藻作用提供参考,也为研究植物化感物质的抑藻机理提 供了新方法. 关键词：iTraq 标记；化感作用；差异表达蛋白组；抑藻机理 中图分类号：X172 文献标识码：A 文章编号：1000-6923(2015)06-1822-09 Differentially expressed proteins in Microcystic aeruginosa with Solidago canadensis L. extracts using iTraq 1 1 1,2* 1 labeling technique. HUANG Ying-ying , BAI Yu , WANG Yan , KONG Hai-nan (1.School of Environmental Science and Engineering, Shanghai Jiaotong University, Shanghai 200240, China；2.School of Public Health, Shanghai Jiaotong University, Shanghai 200025, China). China Environmental Science, 2015,35(6)：1822~1830 Abstract ：To investigate antialgal mechanism of Solidago canadensis L. extracts, iTraq labeling technique coupled with LC-MS-MS was used to analyze differentially expressed proteins in Microcystic aeruginosa. 261 differentially expressed proteins (fold change ratio≥ 1.5) were identified, in which 220 proteins were upregulated and 41proteins were down-regulated. GO function analysis showed that the extracts of S. canadensis inhibited the algal growth by affecting metabolism process, protein catalytic activity and binding function. KEGG pathway analysis showed that top 3 enrichment pathway classes were all related to metabolism, including energy, carbohydrate and amino acid metabolism, and glycolysis/gluconeog