1白藜芦醇通过上调sirt1促进退变椎间盘终板 - 第三军医大学学报.docVIP

白藜芦醇通过调节SIRT1活性促进退变椎间盘终板软骨细胞 钟小明 胡侦明 沈皆亮 王大武 甘强 郝杰 (重庆医科大学附属第一医院骨科，重庆，400016) 摘要: 目的　对的。方法　分为组 ,即白藜芦醇组、二甲基亚砜对照组、尼克酰胺组及对照组。后type II Collagen，COLA2)、蛋白聚糖聚合物（aggrecan）及SIRT1 mRNA表达水平结果 　干预 SIRT1 mRNA 及 SIRT1蛋白的表达COLA2、aggrecan）的合成；Nam及siRNA干预分别有效抑制了SIRT1酶活性及SIRT1 mRNA 的表达。结论　 关键词: 白藜芦醇 SIRT1细胞 Resveratrol promote extracellular matrix synthesis in degenerative endplate cartilages by activating SIRT1 Zhong Xiaoming , Hu Zhenming , Shen Jieliang , Wang Dawu ,Gan Qiang ,Hao Jie （Department of Orthopaedics, The First Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, P.R. China,） Abstract Objective To explore the effect and mechanism of resveratrol on extracellular matrix synthesize in degenerative disc endplate cartilage cells. Method Degenerative disc endplate chondrocytes(DECs) ,derived from elder patient with lumbar disc hernia, were randomly divided into 5 groups, namely, resveratrol ( Res) group, dimethyl sulfoxide (DMSO) control group, resveratrol +nicotinamide (Nam) group, resveratrol +siRNA group and control group. DECs were stimulated with corresponding reagent for 48 hours. Expression levels of SIRT1,type Ⅱcollagen , aggrecan and SIRT1 mRNA were detected ,as well as enzymatic activity of SIRT1. Result 100μmol/L Res intervention could increase the expression levels of both SIRT1 and SIRT1 mRNA, enzymatic activity of SIRT1 , thus stimulating synthesis of extracellular matrix .Nam and siRNA intervention effectively supress SIRT1enzymatic activity and SIRT1 mRNA expression respectively，thus inhibiting synthesis of extracellular matrix. Conclusion Res intervention could stimulate DECs to synthesize of extracellular matrix, thus inhibit cartilage endplate degeneration. This may take effect by activating SIRT1. Keywords: Resveratrol, SIRT1 , endplate chondrocytes , degeneration ,Collagen Ⅱ、aggrecan Supported by the program of National Science Foundation of China Corresponding Author: Hu Zhenming , M.D., Ph.D.