丹参酚酸与牛血清白蛋白结合反应的特性和结构相关性研究.pdfVIP

中草药 Chinese Traditional and Herbal Drugs 41 8 2010 8 12 1 * 邹晓丽, 王湘敏, 郭海方, 许 卉 , 刘珂 ( , 264005) : 4 A B( SA ~ ) ( BSA) SABSA ( K A ) ( n) ( E) ( r) GH S 4 BSA , BSA A ( SA !) BSA , 3 BSA SABSA [ Y= lg( K A ∀ E ∀ n/ r) ] , SA !# SA # SA ∃# SA , , (clogP) (tPS A/ M r) : ; ; ; ; : R28602 : A : 2010) 08127 104 Characteristic of salvianolic acid binding to bovine serum albumin and structureperformance relationship ZOU Xia li, WAN G Xiangmin, GU O Haifang , XU Hui, LIU Ke ( Sch l f Pharmacy, Yantai Universit y, Y antai 264005, China) Abstract: Objective T study the characteristic f salvian lic acid ( SA) binding t b vine serum albu min ( BSA) and the structureperf r mance relati nship Methods The interacti ns betw een BSA and f ur natur al SA ( SA , r smaric acid; SA ∃, lith speric acid; SA !, salvian lic acid A; and SA , salvian lic acid B) w er e investigated by flu rescence and ultr avi let spectr sc py Results The intr insic flu rescence f BSA w as quenched by SA via f rming SABSA c mplex es and n nradiati n ener gy transfer The parameters f SABSA binding pr cess, such as the static apparent ass ciati n c nstant K A , the number f binding site n, the efficiency f energy tr ansfer E, the spatial distance r w er e btained, and the therm dy namic c nstants G, H , and S w ere calculated Conclusion T he results indicate that SA ! b unds t BSA mainly thr ugh a hy dr ph bic f rce and the ther three SABSA interacti ns are mainly driven by hy dr gen b nd and Van der W aals% f rce Y, a c mprehensive binding par ameter c nstructing fr m the equati n Y= lg ( K A ∀E ∀ n/ r) , c uld be used t reflect the interacti n extent f SABSA sy stem T