生物学中荧光共振能量转移的研究应用进展 - core.pdfVIP

生 物 技 术 通 讯 LETTERS IN BIOTECHNOLOGY Vol. 12 No. 3 A g.2001 S31 * 谢小燕 夏宁邵 ( 361005) 荧光共振能量转移(FRET) 可用于对生物大分子之间的距离进行定 、定量检测, 所采用的材料、方法在近年 都有了很大的发展, 在核酸、蛋白质、细胞器结构功能检测、免疫测定、配体2 受体相互作用测定等方面都有巧妙而有 效的应用, 应用前景十分广阔。 荧光共振能量转移, 分子间距离, 生物检测 Pr ogr ess of th e studies and application s of fluor escence r esonan ce ener gy tr ansfer in th e field of biology XIE Xiao2Yan, XIA Ning2Shao (The Key Laboratory of the Mimistry of Education f or Cell Biology and Tumor Cell Engineering , Xiamen University , Xiamen 361005, P . R. China ) Abstr act The phenomenon of fl orescence resonance energy transfer(FRET) can be tilizedto eval ate or to detect the distance between macromolec les.Recently,many new material and methods have been introd ced into the se of this phenomenon,which has facilicated the str c t re and f nction st dy of n clear,protein, cell lar orgams and the interaction between ligand and recep2 tor.All the application of FRET implys that it has a bright f t re. Key wor ds fl orescence resonance energy transfer, distance between two molec les, biological assay 6 6 6 生物学的发展一向有赖于各种物理、化学研究手段的提 就是能量传递效率 E= R P( R + R ), 其中R 为能量传递 0 0 0 高和迅速渗入, 对于分子生物学 说, 分析手段的进步更是 达到50% 的距离, 它依赖于供受体双方的光物理性质以及 阐明机理、应用开发的必要。为了研究生物大分子的结构和