南极磷虾脱脂蛋白肽抗疲劳和耐缺氧实验研究 - 食品科学.pdfVIP

310 2011, Vol. 32, No. 11 食品科学 ※营养卫生 徐 恺，刘 云，王亚恩，李立德，姜国良* ( 中国海洋大学海洋生命学院，山东 青岛 266003) 目的：通过灌服南极磷虾脱脂蛋白肽研究其对小鼠抗疲劳、耐缺氧能力的影响。方法：对照组小鼠灌 胃蒸馏水，低剂量组、中剂量组和高剂量组分别以南极磷虾脱脂蛋白肽灌胃，灌胃剂量分别为0.2、1.0、5.0g/(kg ·d)， 连续 28d 。分别测定小鼠负重游泳时间和血乳酸(LA) 、血尿素氮(BUN) 、乳酸脱氢酶(LDH) 、肝糖原含量；常压 耐缺氧时间及耗氧量、亚硝酸钠中毒存活时间。结果：高剂量组小鼠负重游泳时间及血清中LDH 含量、LA 清除率及 肝糖原含量明显高于空白组，BUN 含量明显低于空白组。高剂量组常压耐缺氧存活时间及亚硝酸钠中毒存活时间明显 高于空白组。结论：南极磷虾脱脂蛋白肽能明显提高小鼠抗疲劳、耐缺氧的能力，具有非常明显的保健作用。 南极磷虾；脱脂蛋白肽；抗疲劳；耐缺氧；小鼠 Anti-fatigue and Anti-hypoxia Functions of Degreased Peptides from Antarctic Krill in Mice XU Kai ，LIU Yun ，WANG Ya-en ，LI Li-de ，JIANG Guo-liang* (College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China) Abstract ：Objective: To explore the anti-fatigue and anti-hypoxia effect of degreased peptides from Antarctic krill in mice. Methods: The mice were randomly divided into four groups including the control group administered with water and three experimental groups administered with degreased peptides from Antarctic krill at doses of 0.2, 1.0, 5.0 g/(kg·d) for 28 consecutive days, respectively. The swimming time and the contents of lactic acid (LA), blood urea nitrogen (BUN) and lactate dehydroge- nase (LDH) in serum, liver glycogen, survival time and oxygen consumption in hypoxia tolerance test, survival time after sodium nitrite poisoning were determined. Results: The degreased peptides from Antarctic krill at high dose could improve the swim- ming time, serum LDH activity, lactate clearance rate and the content of liver glycogen and decrease BUN in serum. Meanwhile, these degreased peptides at high dose could also increase the survival time in both hypoxia tolerance test and sodium nitrite poisoning experiment. Conclusion: The degreased pept