大鼠虚热证模型的建立、评价和知母对虚热证大鼠影响的研究.pdfVIP

提 要 目的：探索应用热性药物复制虚热证模型大鼠的造模方法及模型评价方法。通过 观察寒性中药知母对虚热证模型大鼠能量代谢及相关指标的影响，分析知母的作用趋 势和规律，探寻反映实验动物寒热属性的指标，揭示其与寒热药物属性的内在联系， 为更好的指导临床用药奠定基础。 方法：用附子、干姜、肉桂水煎液灌胃 14 天复制虚热证大鼠模型，给予知母水 煎液灌胃治疗 7 天，观察虚热证模型大鼠的一般状况、体温、寒热趋向等的变化；应 用代谢笼收集大鼠 24 小时食量、饮水量、尿量和粪量；应用氧弹热量计检测大鼠摄 入能、消化能、粪便能、可代谢能；采用酶联免疫吸附法检测大鼠血浆 T3、T4 含量； 检测空腹血糖含量；检测肝组织糖原、乳酸含量、ATP 酶活力；采用高效液相色谱法 检测肝组织 ATP 含量及肝细胞能荷。 结果：造模结束，模型动物体重明显减轻，T3、T4 明显升高，趾温明显降低； PLS 分析显示虚热组与空白对照组样本点完全分离，得到明显区分。治疗结束，知 母可以显著降低虚热模型 T3、T4 含量、尿量、饮水量、趾温；明显升高空腹血糖。 结论：热性药物附子、干姜和肉桂配伍可成功复制大鼠虚热证模型。寒性中药知 母能降低虚热模型大鼠能量代谢，改善大鼠虚热状态，其药理作用的发挥同降低肝脏 能量代谢和减弱甲状腺激素的分泌有关。血浆甲状腺激素的含量、体重、饮水量、尿 量、空腹血糖含量等指标可以作为寒热证模型以及寒热性中药研究的重要评价指标。 关键词 中药四性；知母；虚热模型；能量代谢 Study on the Establishment and Evaluation of deficient heat syndrome in Rats and the Influence of Common Anemarrhena on this Model Speciality: Research on microcirculation of brain and tumor in the field of integrated TCM and Western Medicine Tutor: Prof. Wang Shijun Author:Cui Shulan Abstract Objective: To establish methods of inducing deficient heat syndrome in Rats and to evaluate the signs and symptoms of the model, and to observe the influence of Common Anemarrhena on changes of energy metabolism and related indexs in rats with deficient heat syndrome. To analyse the tendency, characteristics of the effects. To explore the objective indexes, that can reflectes Cold and Heat Property of experimental animal. To provide useful knowledge for clinical medicine. Methods: In order to induce deficiency-heat model in rats, decoction of three Chinese medicinal herbs with hot property, aconite, dried ginger and cinnamon were administered intragastrically to rats for 14 days. After the model was successful