原创力文档Analysis of α-keratins in the horns of rhinoceros and buffalo by non-native capillary isoelectric focusing：（分析的α-keratins角犀牛和水牛的非毛细管等电点聚焦）.pdfVIP
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Analysis of -Keratins in the Horns of Rhinoceros and Buffalo
by Non-Native Capillary Isoelectric Focusing
T.-L. Huang 1. / P. Yu 2
1Beckman Instruments, Inc., 2500 Harbor Blvd., Fullerton, CA 92634, USA
2Beckman Instruments (Taiwan) Inc., 8F, 216 Tun Hwa Rd., Sec. 2., Taipei 106, Taiwan, ROC
selling and smuggling horns from this endangered ani-
Key Words mal.
Capillary isoelectric focusing Owing to the merit of on-column detection and the ca-
Horn of rhinoceros and buffalo pability of attaining high-resolution separation compa-
~-Keratins rable to the conventional isoelectric focusing, capillary
Urea isoelectric focusing (CIEF) method has recently at-
tracted considerable attention as a fast analytical tool
for profiling of biomolecules [2-3]. The use of narrow
Summary diameter (25-50 gm ID) capillary with CIEF further of-
fers the advantage of minute sample requirement (ng).
A capillary isoelectric focusing (CIEF) method under In the present work, a non-native CIEF method was es-
denaturing condition has been developed for separation tablished to analyze a-keratins, the biological consti-
of a-keratins, highly cross-linked biomacromolecules, in tutes in the horns of rhinoceros
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