骨髓增生异常综合征恶性转化过程中c-myc和MMP-9表达的变化及其意义.pdfVIP

· 22· 滨州医学院学报2008年2月第31卷第1期 BMU Journal，Feb．2008，Vo1．31，No．1 骨髓增生异常综合征恶性转化过程中c—myc和 MMP．9表达的变化及其意义 黄延新 叶 翎 1山东省医学科学院 济南市 250062；2济宁医学院附属第一人民医院 【摘要】 目的 探讨c-myc和MMP-9在骨髓增生异常综合征(MDS)恶性转化中的意义。方法 采用逆转录．聚合酶链反应 (RT—PCR)技术检测c—myc mRNA和MMP-9 mRNA在MDS低危(RA，RARS)、高危(RAEB，RAEB—t)及MDS—AML变组患者骨 髓单个核细胞中的表达，并与10例正常对照进行比较。结果 MDS高危组、MDS—AML变组c—myc表达高于MDS低危组及正 常对照组，差异有统计学意义(P0．05)，高表达c—myc的MDS患者易转化成急性白血病。MMP-9在MDS中的表达与正常对 照差异无统计学意义(P0．05)，但3例骨髓原始细胞数在70％以上的MDS—AML变患者骨髓仍高水平表达MMP-9。结论 c—my在MDS的发展、转化过程中起重要作用，并对判断预后有一定的临床意义；MMP-9在MDS恶性转化中的作用有待进一 步研究。 【关键词】 骨髓增生异常综合征；c．myc基因；基质金属蛋白酶-9；美法仑 【中图分类号】 R322．34 【文献标识码】 A 【文章编号】 1001-9510(2008)01-0022-03 Expression and significance of c-myc and MMP-9 in myelodysplastic syndromes progression HUANG Yanxin YE Ling 1 Shandong Academy Medical Sciences，Jiban 250062；2 Affiliated First Hospital of Jining Medical College 【Abstract】 Objective To investigate the role of MMP-9 and c—myc in the transformation from myelodysplastic syndromes(MDS)to acute myeloid leukemias(AML)．Methods The expression of MMPO and c—myc in bone marrow mononuclear cells(BM—MNCs)of patients with low—risk stage of myelodysplastic syndromes(RA，RARS)，high—risk stage of myelodysplastic syndromes(RAEB，RAEB—t) acute myeloid leukemia secondary to MDS and normal control were examined by using the revert transcription polymerase chain reaction (RT—PCR)method．Results The expression level of c—myc in BM—MNCs of patients with RAEB or RAEB—t were significantly higher than that in RA or RARS(P0．05)，and displayed an increasing tendency with transformation to leukemia．The expression level of MMP-9 mRNA had no significant changes(P0．05)，however three patients with acute myeloid leukemia secondary to MDS，in which the ratio of blast cells exceed 70％ ，also presented high expression level of