山西省中部地区主栽小麦品种的分子标记分析.pdfVIP

中国农学通报 2010,26(17):20-25 Chinese Agricultural Science Bulletin 山西省中部地区主栽小麦品种的分子标记分析 裴自友，温辉芹，王 晋 ( 山西省农业科学院作物遗传研究所，太原030031) 摘 要：山西省中部地区是山西小麦生产的重要地区，了解该区当前主栽小麦品种的基因组成特点，对 于进一步选育新品种具有指导作用。采用SDS方法分析了10 个小麦品种的高分子量谷蛋白亚 基和醇溶蛋白组成，并利用5 个功能基因标记分析了其矮秆基因、硬度、多酚氧化酶等基因组成。结果 表明，供试材料HMW-GS 存在4 种组合类型，其中晋太9923 亚基组合为1，7+8 和5+ 10，评分为10分；根 据醇溶蛋白和黑麦专化标记分析说明4 个品种含有T 1RS ·1BL 易位；3 个品种含Rht2 ，7 个品种含Rht8， 1 个品种为Rht2+Rht8 组合；4 个品种扩增出Pinb -D 1b 位点，5 个品种具有Pinb -D 1a 位点，1 个品种为杂合 型；利用多酚氧化酶基因标记在10 个品种检测出3 个不同位点，表明该基因在品种间存在多样性，其中 4 个品种含有长度为876 bp 的低活性位点。 关键词：小麦；品质；农艺性状；标记分析 中图分类号：S512 文献标志码：A 论文编号：2010-0477 Molecular Marker Analysis on Major Wheat Cultivars in Central Shanxi Province Pei Ziyou, Wen Huiqin, Wang Jin （Institute of Crop Genetics, Shanxi Academy of Agricultural Sciences, Taiyuan 030031) Abstract: The central region of Shanxi Province is very important for wheat production in this province. Understanding genetic constitutions of these cultivars will provide useful information for wheat breeding. Using SDSand functional markers of five wheat genes, the high molecular weight glutenin subunits and genetic constitutions of 10 major cultivars in the area were analyzed. The results showed that the 10 cultivars included four types of HMW-GS combinations, of them, Jintai 9923 have a combination of 1, 7 +8 and 5+ 10. Analyzed with secalin and 1RS specific markers, four cultivars carried T 1RS ·1BL translocation. Three cultivars carried Rht2, 7 carried Rht8, one carried both Rht2 and Rht8. Four cultivars carried Pinb-D1b, five cultivars carried Pinb-D1a. Using PPO gene specific marker, three loci were revealed in the ten cultivars indicating genetic polymorphism,