银杏黄酮甙对糖尿病大鼠肾组织PAI-1、MMPs-3表达的影响.pdfVIP

第25卷 第1期 河 北 职 工 医 学 院 学 报 V01．25 No．1 2 0 0 8年2月 Journal of Hebei Medical College for Continuing Education Feb．2008 银杏黄酮甙对糖尿病大鼠肾组织 PAI一1、MMPs一3表达的影响 侯淑芬 ，刘秋菊 ，张立新 ，张春雪 ，赵芬宣 ，晋素景 (1．河北大学附属医院 肾内科，河北保定 071000；2．河北大学医学部 电教中心，河北保定 071000) 摘要：目的探讨银杏黄酮甙对糖尿病大鼠肾脏保护作用机制。方法选择雄性sD大鼠48只，随机分为3组，每组l6只，即正 常对照组(A组)，糖尿病组(B组)，银杏黄酮甙治疗组(C组)。B、C组腹腔注射链脲佐菌素(STZ)制模成功后，C组给予银杏 黄酮甙灌胃，l0周后将大鼠宰杀，用免疫组化方法观察肾脏PAl一1、MMPs一3的表达。结果与A组相比免疫组化：PAl一1第6 周时B、C组均高于A组(P0．01，P0．05)，持续至第l0周(P0．01)，但C组低于B组(P0．01)。MMPs一3第6周时B组 低于A组(P0．05)，持续至第l0周(P0．01)，C组则高于A、B组(P0．01)。结论STZ诱导的糖尿病大鼠PAl一1表达增 强、MMPs一3表达下调。应用银杏黄酮甙治疗，可以使PAl一1的表达下调，MMPs一3的表达上调，对糖尿病大鼠肾脏有保护 作用。 关键词：糖尿病肾病；银杏黄酮甙；血浆纤溶酶原激活物抑制物(PAt一1)；基质金属蛋白酶一3(MMPs一3) 中图分类号：R一332 文献标识码：A 文章编号：1008—6692{2008}01—0010—02 Effects of Ginkgo Flavonal on the expression of PAI一1、MMPs一3 in diabetic rats Hou Shufen Liu Qinju Zhang Lixing2 et．a1． (Department ofNephropathy，Affiliated Hospital ofttebei University，Baoding，071000) Abstract：Objective To investigate the role of Ginkgo Flavonol on the mechanism of protecting kidney in diabetic rats． Methods：Male Sprague—Dawley rats weighting 1 80—200g were randomly divided into thme groups：the control rats (Group A)，the diabetic rats(Group B)and the diabetics rats treated with Ginkgo Flavonol(Group C)．Streptozotoein (STZ)65mg／kg body weight，were celioinjected in Group B and Group C．Group C was treated with Ginkgo flavono1．6 weeks and 10 weeks after the treatment with Ginkgo flavolon，the following studies were performed in every group．The renal tissue dealt with formalin were obtained，Immunohistochemieal technique(SP assay)was used to estimate the ex— pression of matrix metallopmteinases一3(MMPs一3)，Plasminogen activator inhibitor一1(PAI一1)in renal cortex and the renal tissue of diabetic rats were observed．Results：The level of renal PAI一1 were higher in Group B、C than in Group A(P0．01，P0．05)，In 10 weeks，its 1eve1in Group