上海地区宠物犬中戊型肝炎病毒流行病学调查.pdfVIP

安徽农业科学，Journal of Anhui A ．Sci．2008，36(14)：5895—5896，5941 责任编辑 张杨林 责任校对 况玲玲 上海地区宠物犬中戊型肝炎病毒流行病学调查 刘俊峰L2，华修国2 7张文2 7李培锋17舰I士v~J )iL 7沈权 (1．内蒙古农业大学动物科学与医学学院，内蒙古呼和浩特 010018；2．上海交通大学农业与生物学院，上海市兽医生物技术重点实验室，上海 200240) 摘要 的]为探讨宠物犬在戊型肝炎病毒(HEV)流行环节中的作用提供依据。[方法]以散养宠物犬为研究对象，从上海地区及其周 边地区随机采集70份血清样品，通过双抗原夹心ELISA试验检测抗HEV抗体，分析不同品种和不同性别宠物犬中血清抗HEV抗 体阳性率。利用反转录巢式PCR检测血清中病毒核酸。[结果]在70份宠物犬血清样品中，15份样品中抗HEV抗体呈阳性，阳性率 为21．4％。上海地区宠物犬阳性率为27．5％，高于上海周边地区。在供试的宠物犬品种中，杜宾犬的HEV抗体阳性率较高(33．3％)，其 他品种宠物犬为11．1％ 25．0％。雌性宠物犬中HEV阳性率为34．4％，显著高于雄性宠物犬。在各种宠物犬中未检测到 HEV核酸颗 粒。『结论l宠物犬对HEV敏感l在HE流行环节中具有不容忽视的作用。 关键词 戊型肝炎病毒；反转录巢式PCR；双抗原夹心酶联免疫试验 中图分类号 $858．292 文献标识码 A 文章编号 0517—6611( 2008)14—05895-02 Epidemiological Investigation on the Hepatitis E Virus in Pet Dogs in Shanghai Area LlU Jun-feng et al (College of Animal Science and Medicine，Inner Mongolia Agricultural University，Hohhot，Inner Mongolia 010018) Abstract [Objective]The research aimed to provide basis for discussing the role of pet dog in the epidemic links of hepatitis E virus，[Method] With scatter-fed pet dog as research ob eets 70 shares of pet dog serum samples were randomly collected from Shanghai area and its surrounding areas．Anti—HEV antibodies were deteeted in double-antigen sand,dich ELlSA test to analyze the positive rate of serum anti—HEV antibodies in different varieties and different sexes of pet dogs，The viral RNA in the serum was detected by using RT-nested PCR．[Resultl Among 70 shares of serum samples of pet dogs．anti—HEV antibodies in 15 sampies showed positive．with the positive rate of 21．4％，The positive rate of pet dogs in Shanghai area was 27，5％．higher than that in the surrounding areas of Shanghai，Among the tested varieties of pet dogs,the HEV positive rate of Dubin dog was higher f33，3％)，while that of other varieties of pet dogs were l1．1％一25．0％．The HEV infection rate of female pet dogs was 34，4％，significantly hig11er than that of male pet dogs，HEV RNA gr