pcr选购常识（PCR buying common sense）.docVIP

pcr选购常识（PCR buying common sense） Selection of quantitative PCR instrument Release time: 2007-9-9 browsing times: 1332 times The history of fluorescence quantitative PCR: Fluorescent quantitative PCR is a kind of PCR. PCR aims for gene amplification. In the gene amplification, the most important is the temperature rise and fall process. The earliest PCR is three water bath pots, a mechanical arm, and the reaction tank is periodically caught and converted into a water bath pan. The results of PCR need to be tested by other means. Later, with the development of quantitative technology, PCR and detection were integrated into a quantitative PCR instrument. Since the fluorescent technology is applied and can be monitored during each amplification process, the standard is called real-time fluorescent quantitative PCR. To be precise, real-time fluorescent quantitative PCR does not have automatic sampling and sample handling systems, but can only be semi-automatic. PCR simply speaking, is a temperature control equipment and a test equipment. Real time fluorescent quantitative PCR technique was first proposed by a Japanese Higuchi report for the first time in 1992, he wanted to see the whole process of real-time PCR reaction at that time, because of the widespread use of EB (EB), think the most direct dye is EB, can be inserted into the double stranded nucleic acid stimulated luminescence, in PCR reaction annealing or extension detection incorporation into the content can be real-time monitoring of PCR double stranded nucleic acid EB in the process of considering the function of the PCR reaction, combined with the corresponding algorithm, through the method of adding standard, it can make precise quantitation of target gene in the samples tested. Thus, the first real-time quantitative PCR instrument was born on the basis of the ordinary PCR instrument and equipped with a device for excitation and detection. And the real market of the United States application, Bio System Inc