169例急性白血病免疫表型分析.pdfVIP

668 实用预防医学 2008年6月 第15卷 第3期 Practical Preventive Medicine，Jun．2008，Vol 15，No．3 文章编号：1006—3110(2008)03—0668—04 【论 著 】 169例急性白血病免疫表型分析 李艳明，曹志红，彭敏源 摘要： 目的 探讨急性白血病的免疫表型特征，为急性白血病的诊断提供依据。 方法 收集169例初治急性白 血病患者骨髓或外周血标本，采用流式细胞仪对其白血病细胞进行免疫表型分析。 结果 95例急性髓细胞白血病 (acute myeloblastic leukemia，AML)患者中59例为纯髓系抗原表达，34例伴淋系抗原(CD19、CD7)表达。64例急性淋巴 细胞白血病(acute lymphoblastic leukemia)患者中62例表达淋系抗原，纯T系抗原表达8例，纯B系抗原表达44例，6例伴 有髓系抗原(CD33)表达；1例ALL—L2为纯髓系(CD33、CD34)表达，1例ALL—L2八个抗原检测均为阴性。10例急性 混合型白血病(mixed acute leukemia，MAL)同时表达两种以上淋系和髓系抗原标志。 结论 免疫分型能助诊一些特殊 类型的白血病(如minimally differentiated acutemydoidleukemia，AML M0；MAL)；免疫分型是对白血病细胞形态学诊断 的重要补充。 关键词： 免疫分型；急性白血病；分化簇；流式细胞仪 中图分类号：R557 文献标识码：A Immunophenotype Analysis on 169 Cases of Acute Leukemia LI Yan ming，CAO Zhi—hong，PENG Min—yuan(Xiang ya Hospital，Central South University，Changsha 410008，Hunan) Abstract： 0bjective To explore the immunophenotypical characteristics for the diagnosis of acute leukemia． Meth- od The immunophenotype of bone marrow or blood samples collected frOm 169 patients with newly diagnosed acute leuke— mia was analyzed by flow cytometer． Results Myeloid antigens were expressed alone in 59 of 95 patients with acute my- eloid leukemia(AML)，34 patients with AML showed aberrant lymphoid antigens(CD19 or CD7)expression．Lymphoid anti- gens were expressed in 62 0f 64 patients with acute lymphoid leukemia(ALL)，and T or B lymphoid antigens were detected alone in 8 or 44 patients respectively．Six patients with ALL showed aberrant myeloid antigens 0D33 expressi0n．In one pa- tient with ALL—L2，only 0D33 and 0D34 were detectable and in another patient with ALL—L2 eight antigens were undetect- able．Ten patients with mixed acute leukemia(MAL)showed lymphoid and myeloid