uplc法测定人血清中雷公藤甲素浓度δ - 中国药房.pdfVIP

UPLC 法测定人血清中雷公藤甲素浓度Δ ＊ 李 颖 ，汪永忠，李 翔，吴 建，夏伦祝（安徽中医学院第一附属医院/ 国家中医药管理局中药制剂三级实验 室，合肥 230031） 中图分类号 R969.1 文献标志码 A 文章编号 1001-0408（2013）12-1083-02 DOI 10.6039/j.issn. 1001-0408.2013.12.09 摘 要 目的：建立测定人血清中雷公藤甲素浓度的方法。方法：采用超高效液相色谱法，内标为艾司唑仑。色谱柱为Waters Ac- quity C18柱，流动相为乙腈-水（30∶70，V/ V），流速为0.2ml/min ，柱温为室温，检测波长为220nm ，进样量为5μl。结果：雷公藤甲素 血药浓度在13.13～840.00ng/ml 范围内同雷公藤甲素与内标物峰面积比值线性关系良好；日内、日间RSD 均＜15％，方法回收率 为88.25％～99.33％。结论：该方法快速、灵敏、准确，可用于雷公藤甲素的血药浓度监测和药动学研究。 关键词 雷公藤甲素；超高效液相色谱法；血药浓度；测定 Determination of Triptolide Concentration in Human Serum by UPLC LI Ying ，WANG Yong-zhong ，LI Xiang ，WU Jian ，XIA Lun-zhu （The First Affiliated Hospital ，Anhui College of TCM/Third Level Laboratory of TCM Preparation ，State Administration of TCM ，Hefei 230031，China ） ABSTRACT OBJECTIVE ：To establish a method for determining the concentration of triptolide in human serum. METHODS ： UPLC method was adopted. The column was Waters Acquity C18 column with mobile phase consisted of acetonitrile-water （30∶70， V/ V）at flow rate of 0.2 ml/min using estazolam as internal standard. The temperature of column was set at room temperature and the detection wavelength was set at 220 nm. The injection volume was 5 μl. RESULTS ：The linear ranges of triptolide were 13.13-840.00 ng/ml ，and RSD of intra-day and inter-day was lower than 15％. The recoveries were 88.25％-99.33％. CONCLU- SION ：This method is rapid ，sensitive and accurate ，and can be used for pharmacokinetic study and serum concentration monitor- ing of triptolide. KEY WORDS Triptolide ；UPLC ；Serum concentration ；Determination 雷公藤多苷是从卫矛科植物雷公藤根提取精制而成的一 工作站（美国Waters 公司）；80-2离心沉淀器（上海手术器械 种极性较大的脂溶性成分混合物，属国家基本药物，临床应用 厂）；XW-80A 旋涡混合器（上海医科大学仪器厂）；BS210S 电 [1] 广泛 。而环氧二萜类化合物雷公藤甲素（Triptolide ）是雷公 子天平（德国赛多利斯公司）；MTN-2800D 氮吹仪（天津奥特赛 藤多苷制剂的质控指标，被认为是其最重要的活性成分和毒