超高效液相色谱串联质谱法检测六种动物组织中盐酸氯丙嗪残留.pdf

20 10, 44 ( 2) : 7 ~ 11/, 7 李丹妮, 黄士新, 张文刚, 张 鑫 ( , 20 1103 ) [ ] 2009- 09 - 0 1 [ ] A [] 1002- 1280( 20 10) 02 - 0007- 05 [ ] S859. 84 [ ] 建立了检测六种动物组织中盐酸氯丙嗪残留的超 效液相色谱- 串联质谱( U PLC / M S /M S)分析方法样品经碱性乙醚提取, 浓缩后用甲醇溶解, 经超 液相色谱- 串联质谱在多 反应检测( MRM ) 模式下测定在 3 m in以内完成盐酸氯丙嗪的定量分析结果显示盐酸氯丙嗪 标准曲线在 0. 2 ~ 100 g /L 浓度范围内线性良好, 标准溶液中D3 - 氯丙嗪内标浓度为固定值 5 g /L线性相关系数( r )为 0. 999 8 在 0. 51510 g /kg添加水平下, 盐酸氯丙嗪的加标回收 率在 83% ~ 104% , 相对标准偏差(RSD ) 均小于 10% ( n = 6) 本方法对六种动物组织中盐酸氯丙 嗪的定量限为0. 5g / kg ( S /N 10) , 检出限为 0. 2 g / kg ( S /N 3) [] 盐酸氯丙嗪 动物组织 残留 超 效液相色谱- 串联质谱法 Determ ination of Chlorpromazine Hydrochloride Residue in Six K inds ofAnimal Tissues by U ltra Performance L i uid Chromatography- Tandem M ass Spectrometry L I D an - N i, HUANG Sh i- x in, ZHANG W en - gang, ZHAN G X in ( Shangha iM un icip al Institute of Veterinary D rug and F eed stuff Con tro,l Shangha ,i 20 1103; China) Abstract: A smi ple, sen sitive and u ltra p erfo rm ance liqu id chrom atography- tandem m ass spectrom etric ( U PLC / M S /M S) m ethod h as been deve lop ed for the qu antification of chlorprom az ine hydrochloride residu e in six k inds o f an mi al tissu es. Chlorprom azine hydroch loride extracted by a lkaline ether from anmi al tissu e, w as fina lly d isso lved in the m eth ano l after the procedure of be ing concen trat ion. T he sam ple w as sep arated on BEH C18 ( 100 mm 2. 1 mm i. d. , 1. 7 m ) co lum n u sing a m ob ile phase of acetonitrile /5 mmm ol/L amm onium acetate. Ch lorprom azine hydrochloride w as detect