猪瘟野毒与疫苗弱毒鉴别方法的研究进展.pdfVIP

20 11, 39( 2) : 138~ 140 Guizh u Agricultural Sciences [ ] 100 1360 1( 20 11) 020111013803 猪瘟野毒与疫苗弱毒鉴别方法的研究进展 1 1* 1 2 2 3 杭柏林 , 胡建和 , 王青 , 史杰 , 李 杰 , 朱王飞 ( 1. , 453003; 2. , 453003; 3. , 225000) [ ] 概述了当前鉴别猪瘟强弱毒的几种方法, 即免疫学方法( 胶体金免疫层析技术单克隆 体法 和单 酶联免疫吸附试验) 核酸检测方法( RTPCR 技术RTPCR 与其他方法的结合) 和动物试验方法 同时提出了鉴别猪瘟强弱毒方法的发展方向 [ ] 猪瘟病毒; 野毒; 弱毒; 鉴别诊断 [ ] S855. 9 [ ] A Research Progress on Differential Methods of Wildtype and Vaccine Virus of Classical Swine Fever 1 1* 1 2 2 3 HANG B lin , HU Jianhe , WANG Qing , SHI Jie , LI Jie , ZHU Wangfei ( 1. Collegeof Animal Science, HenanInstitute of Sicenceand Technology, Xinxiang, Henan, 453003; 2.Xinxiang Monitor and Inspection Center f or uality of Livestock Products, Xinxiang, Henan 453003; 3. Yangzhou EntryExit Inspection and uarantineBureau, Yangzhou, Jiangsu 225009, China) Abstract: This paper mainly summar izes differential meth ds f w ildtype and vaccine virus f classical sw ine fever, w hich are immun l gy meth ds( GICA, M cA b and McAbELISA ) , nucleic acid detecti n meth ds( RTPCR and c mbinati n appr ach f RTPCR w ith ther meth ds) and animal test meth d. And the p tential devel pment directi ns f differential meth ds f w ildtype and vaccine virus f classical sw ine fever are als pr p sed in this paper. Key words: classical sw ine fever virus; w ildtype virus; vaccine virus; differential diagn sis [ 7] ( Classical sw ine fever vi rus, CSFV) , , [ 1] , , [ 8]