高效液相色谱法检测饮料中7种人工合成色素 - 食品安全质量检测学报.docVIP

高效液相色谱法检测饮料中7种人工合成色素代弟, 徐 静, , 苏明明, 宋雪冬, 曹际娟辽宁出入境检验检疫局, 大连116001) 摘 要: 目的建立饮料中7种人工合成色素柠檬黄、新红、苋菜红、胭脂红、日落黄、亮蓝、赤藓红的高效液相色谱法检测方法。方法样品中的7种人工合成色素在pH=6的条件下经1.0%的乙腈水提取, 超声, 离心后供液相色谱检测。检测波长为420、520、650 nm, 外标法定量。结果7种人工合成色素在1.0~50 μg/mL浓度范围内线性关系良好, 相关系数均大于0.9981, 平均回收率为73.6%～107.2%, RSD为7.8%～14.77%, 检出限均为5 mg/kg。结论本方法操作简便、经济实用、结果准确可靠, 可用于样品的批量检测。 关键词人工合成色素; 高效液相色谱法; 饮料Determination of 7 kinds of synthetic pigments in beverage by high performance liquid chromatography DAI Di, XU Jing, YANG Lu, SU Ming-Ming, SONG Xue-Dong, CAO Ji-Juan* (Liaoning Entry-Exit Inspection and Quarantine Bureau, Dalian 116001, China) ABSTRACT: Objective To determination of 7 kinds of synthetic pigments (including tatrazine, new red, amaranth, carmine, sunset yellow, brilliant blue and erythrosine) in beverage by high performance liquid chromatography (HPLC). Methods The 7 kinds of synthetic pigments in samples were extracted by 1.0% acetonitrile (pH=6) solution and ultrasonic. After centrifuged, the extractions were determined by HPLC at 420, 520, and 650 nm. The external standard method was used for quantitative analysis. Results The method showed a good linearity in the range of 1.0~50 μg/mL for 7 kinds of synthetic pigments with correlation coefficients above 0.9981. The recovery rates, relative standard deviations (RSDs) and detection limits of this method were 73.6%～107.2%, 7.8%～14.77% and 5 mg/kg, respectively. ConclusionThe method is simple, economical and practical with accurate and reliable results, which is suitable for batch samples determination. KEY WORDS: synthetic pigments; high performance liquid chromatography; beverage  1 引言 随着科技的发展和人民生活水平的提高, 食品中的色素的使用量日趋增加。色素分为两种, 一种是天然色素, 另一种是人工合成色素。天然色素来源于动物和植物, 对人体危害较小, 但天然色素价格高, 在食品加工、贮存过程中容易褪色和变色。合成色素成本低廉, 色泽鲜艳, 性质稳定, 易于调色, 着色力强, 使用方便, 已在食品工业中得到广泛使用[1]。然而, 经复杂工艺过程, 合成色素易混入有害物质, 过量摄入合成色素甚至会损害人体健康。目前我国批准使用食用合成色素都有最大使用限量[2], 因此食品中的人工合成色素的检测具有十分重要的意义。目前, 对食品中的人工合成色素的检测的方法有GB/T 5009.35-2003[3], 然而此方法过于繁琐, 消耗的试剂多, 样品处理步骤复杂, 在样品批量检测时, 消耗大量的人力物