玉米热激蛋白基因zmhsp90-1 的克隆及表达分析 - 作物学报.pdfVIP

作物学报 ACTA AGRONOMICA SINICA 2012, 38(10): 1839− 1846 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2012.01839 玉米热激蛋白基因ZmHsp90-1 的克隆及表达分析 刘玲玲1,2 柳思思2,4 翁建峰2 王昌涛3 李新海2 张世煌2 石庆华4 王丽娟1,* 郝转芳2,* 1 , 150030; 2 / , 100081; 3 , 100037; 4 , 830052 : Hsp90 Hsp90 , ZmHsp90-1 , cDNA 2 371 bp, 2 094 bp, 697 , 79.98 kD , ZmHsp90-1 ATPase Hsp90 , ; ZmHsp90-1 AtHsp90.1 , 88.3%, ZmHsp90-1 PCR , ZmHsp90-1 ABA ZmHsp90-1 : ; ; ; ZmHsp90-1 Cloning and Expression Analysis of Heat Shock Protein Gene ZmHsp90-1 in Maize 1,2 2,4 2 3 2 2 LIU Ling-Ling , LIU Si-Si , WENG Jian-Feng , WANG Chang-Tao , LI Xin-Hai , ZHANG Shi-Huang , SHI Qing-Hua4 , WANG Li-Juan1,*, and HAO Zhuan-Fang2,* 1 College of Life Science, Northeast Agricultural University, Harbin 150030, China; 2 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / National Engineer Laboratory of Crop Molecular Breeding, Beijing 100081, China; 3 Beijing Technology and Business University, Beijing 100081, China; 4 College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China Abstract: The heat shock protein 90 (Hsp90) is a widespread family of molecular chaperones found in prokaryotes and all eu- karyotes, with high conservation among plant species. In this study, a Hsp90 gene was isolated from maize, named ZmHsp90-1. The full cDNA sequence of ZmHsp90-1 is 2 371 bp, containing a 2 094 bp open reading frame (ORF) and encoding 697 amino acids with a predic