龙胆合剂治疗湿热型湿疹的疗效观察和其抗Ⅳ型变态反应的实验研究.pdfVIP

提 要 目的:评价龙胆合剂治疗湿热型湿疹的疗效；观察龙胆合剂对IV型变态反应的影 响，探讨龙胆合剂治疗湿疹可能的作用机理。 方法：临床研究：将60例辨为湿热型的湿疹患者，随机分为2组，即治疗组（龙 胆合剂）和对照组（龙胆泻肝软胶囊），均疗程四周。治疗后观察比较两组的临床疗 效。实验研究：取50只小鼠，建立小鼠IV型变态反应模型，随机分为五组，分别灌胃 予生理盐水、龙胆合剂高、中、低浓度、强的松，观察比较各组小鼠耳廓肿胀度；另 取10只小鼠不用DNCB诱发皮炎,仅用生理盐水作为正常对照，观察比较各组CGRP的浓 度。 结果：治疗后治疗组的治愈率为66.67%，总有效率为100%；与对照组比较，治愈 率有显著性差异（P0.05），总有效率无显著性差异（P0.05）。实验显示：龙胆合 剂可明显抑制IV型变态反应。 结论:龙胆合剂能显著改善患者红肿、瘙痒等症状，对湿热型湿疹疗效确切，复 发率低，其作用机理可能与抗IV型变态反应等有关。 关键词 湿疹；龙胆合剂；临床研究；实验研究 The Clinical and Experimental Study on Treating Damp-Heat Eczema by LongDan Mixture Speciality:Dermatology,Surgery of TCM Author:Yang Cuicui Tutor:Professor Du Xixian Abstract Objective: To observe clinical efficacy for treating Damp-heat eczema and impact on type IV allergy by LongDan Mixture(LDM); explore the therapy mechanism of LDM in Damp-heat eczema. Method: clinical research: 60 patients were separated at random into group with LDM and group with soft capsule of LongDanXieGan. During 4 weeks’treatments, the clinical efficacy were compared. In the animal experiment,Ⅳ allergic reaction model was constructed. After devided into group at random, saline, prednisone and LDM were given respectively. The ears weight difference of the mice in the five groups was observed.Another 10 mice do not have DNCB-induced dermatitis,only normal saline were used as normal control. The diffierent group of CGRP concentration was observed. Result: Compared with group with soft capsule of LongDanXieGan, the cure rate is 66.67% with total effect rate of 100% and the cure rates of two groups had significant difference (P0.05 ), the total effect rate of no significant difference (P0.05 ). The experiment shows that LDM can remarkably restrain Ⅳallergic reaction. Conc