烤烟富钾基因型钾吸收积累与内向钾电流特性-生物通.DOC

烤烟基因型钾吸收积累与内向钾电流特性杨铁钊鲁黎明，夏巍，范进华 （河南农业大学农学院，郑州 450002）·L-1时，烤烟富钾基因型ND202叶片平均干重及总生物学平均干重分别为10.20 g及14.85 g，均高于净叶黄（JYH）（分别为8.50 g及13.11 g）和NC2326（分别为8.39 g及12.72 g）。当外界供钾水平大于0.5 mmol·L-1时，ND202叶片钾的积累量比净叶黄（JYH）高18.6%，比NC2326高34%。同时，ND202的Vmax为118.11 μmol·g-1 FW·h-1，显著高于净叶黄（JYH）的58.87 μmol·g-1 FW·h-1及NC2326的64.40 μmol·g-1 FW·h-1。在根皮层内向钾电流方面，ND202的电流密度（pA/pF）的最大绝对值为60，也明显高于净叶黄（JYH）（50）及NC2326（40）。【结论】叶片钾积累量、叶片相对含钾量、Vmax以及根皮层内向钾电流，可以作为富钾烤烟基因型筛选的参考指标。 关键词：烤烟；富钾基因型；钾吸收；含钾量；内向钾电流 Characteristics of Potassium-Enriched Flue-Cured Tobacco Genotype in Potassium Absorption, Accumulation and In-Ward Potassium Currents of Root Cortex YANG Tie-zhao, LU Li-ming, XIA Wei, FAN Jin-hua (College of Agronomy, Henan Agricultural University, Zhengzhou 450002) Abstract: 【Objective】The purpose of this experiment is to the characteristics of potassium-enriched flue-cured tobacco genotype in potassium absorption, accumulation and in-ward potassium currents of root cortex.【Method】Hydroponic methods, K+-depletion methods and patch-clamp whole-cell recordings were carried out to investigate the accumulation of dry matter and potassium in different organs, characteristics of dynamic of potassium absorption and in-ward potassium currents in potassium-enriched flue-cured tobacco genotype and control.【Result】The average dry weights of leaves and whole plant of potassium-enriched flue-cured tobacco genotype ND202 were 10.20 g and 14.85 g, respectively, higher than JYH (8.50 g and 13.11 g) and NC2326 (8.39 g and 12.72 g), when potassium concentration in the solution varied between 0.1 and 50 mmol·L-1. Potassium accumulation in leaves of ND202 was 18.6% more than JYH and 34% more than NC2326, when potassium concentration in the solution higher than 0.5 mmol·L-1. The Vmax of ND202 was 118.11 μmol·g-1FW·h-1, obviously higher than that of JYH, 58.87 μmol·g-1FW·h-1, and NC2326, 64.40 μmol·g-1 FW·h-1. As for the in-ward potassium currents, the absolute value of current density (pA/pF) of ND202 was 60, higher