天幕毛虫核型多角体病毒对天幕毛虫幼虫的致病机理.pdfVIP

第36卷第3期 东 北 林 业 大 学 学 报 V01．36 No．3 2008年3月 JOURNAL OF NORTHEAST FORESTRY UNIVERSITY Mar．2008 天幕毛虫核型多角体病毒对天幕毛虫幼虫的致病机理 ’ 胡春祥 张志强 ． (东北林业大学，哈尔滨，150040) 摘 要 用天幕毛虫核型多角体病毒(Nucleus polyhedrosis virus，NPV)感染天幕毛虫(Malacosoma neustria testacea Motschulsky)幼虫，对其致病机理的研究表明：该病毒可以侵染和破坏天幕毛虫的中肠细胞，在中肠组织细 胞中均能观察到形成的多角体。幼虫取食核型多角体病毒100 h后，在光学显微镜下可见细胞核内出现多角体形 成。细胞核开始增大；取食130 h后，中肠组织细胞间出现缝隙，细胞核内多角体数量增多；取食160 h后，中肠细胞 的细胞核进一步增大，个别细胞核增大至整个细胞的1／3以上，与正常天幕毛虫幼虫相比，整个虫体出现软化；取 食190 h后，中肠细胞受到严重破坏，细胞结构模糊不清，中肠内食物极少并有许多游离的核型多角体存在；取食 220 h后，天幕毛虫幼虫的中肠和其他组织完全受到破坏。 关键词 天幕毛虫；核型多角体病毒；幼虫；致病机理 分类号 Q965．9 Pathogenesis ofNudear Polyhedrosls Vh-us to the Lsrvae ofMalacosoma nd testacea Motschulsky／Hu Chunxi~g． Zhang Zhiqiang(College of Forestry，Northeast Forestry University，Iq【arbin 150040，P．R．China)／／Journal of Northeast Forestry University．一2008．36(3)．一65—67 An experiment was conducted to infect Malacosoma neustria testacea Motschulsky larvae with nuclear polyhedrosis vi． rUS(NPV)．Result indicated that the NPV could invade and damage the midgut cells of the larvae，and polyhedra were found in the midgut cells．The polyhedron formation was detected in the karyon under microscope and the karyon began swelling 100 hours after the larvae fed NPV；gaps appeared between midgut ceils and the polyhedra increased in karyon af- ter 130 hours：the karyons of midgut cells swelled further，individual karyon swelled to more than 1／3 0f the whole cell， the body of the larvae becam e softened compared with the norm al larvae after 16O hours：the midgut cells were seriously dam aged，the cell structure blurred，food in midgut Was very litde and there were a lot ofdissociative pelyhedra after 190 hours：the midgut and other tissues of the larva