盐酸甲氯芬酯联合埋线对血管性痴呆大鼠学习记忆的影响.pdfVIP

第33卷 第1期 贵 阳 医 学 院 学 报 VOI．33 NO．1 2008年2月 JOURNAL OF GUIYANG MEDICAL COLLEGE 20o8．2 盐酸甲氯芬酯联合埋线对血管性痴呆大鼠学习记忆 的影响 陈 粲，杨 琼，戴桃李，潘 娅，王旭东，张 敏 (贵阳医学院基础医学院生理学教研室，贵州贵阳 550004) [摘 要】目的：研究盐酸甲氯芬酯联合穴位埋线对血管性痴呆(VD)大鼠海马神经细胞及学习记忆的影响。 方法：改良Palsinelli四血管阻断法建立血管性痴呆大鼠模型，在造模后给予盐酸甲氯芬酯和穴位埋线治疗。采 用Morris水迷宫检测学习记忆能力；用HE染色观察大鼠海马齿状回神经细胞的变化。结果：经盐酸甲氯芬酯 联合穴位埋线作用后，VD大鼠齿状回细胞损伤减少；学习记忆能力明显提高，比盐酸甲氯芬酯或穴位埋线单独 作用明显。结论：盐酸甲氯芬酯联合穴位埋线能保护VD大鼠海马神经细胞，明显提高其学习记忆能力。 [关键词】痴呆，血管性；海马；齿状回；埋线疗法；大鼠，Sprague．Dawley；盐酸甲氯芬酯；学习记忆 [中图分类号]R338．8；R743．3 [文献标识码]A [文章编号]1000．2707(2008)01-0026-03 Effects of Centrophenoxine Combined with Point Thread Embedding on the Learning and Memory of the Vascular Dementia Model Rats CHEN Can，YANG Qiong，DAI Taoli，PAN ya，WANG Xudong，ZHANG Min (Department ofPhysiology，Guiyang Medical College。Guiyang 550004，Guizhou，China) [Abstract]Objective：To explore the effects of centrophenoxine combined with point thread embed- ding on the nerve cells in hippocampus and on the learning and memory of the vascular dementia (VD)model rats．Methods：VD animal models were established with rats by employing improved Pulsinelli’S four-vessel occlusion method ，and the rats were then treated with centrophenoxine and point thread embedding．Morris maze test was used to evaluate the learning and memory of the rats， and the nerve cells in hippocampus was detected by observing HE stained sections．Results：After the combined treatment of centrophenoxine and point thread embedding，the number of injured neurons in the dentate gyms area of the hippocampus decreased，and the learing and memory of the VD model rats were improved significantly．The results were better than those when centrophenoxine or point thread embedding was used alone．Conclusion：Centmphenoxine combined with point thread